通过建立能够更好地模拟体内环境的人类细胞和组织的体外模型，能防止许多这样的死亡。
Many of these deaths could be prevented through the development of in vitro models of human cells and tissues that better mimic in vivo environments.
我们首先得做一个体外细胞培养实验，在动物模型中证明它的存在，然后开发出一种能影响突变基因产物的分子或蛋白质。
We have to do in-vitro cell culture experiments first, prove it in an animal model, and then develop a molecule or protein which will affect the mutated gene product.
EN的建立，为深入研究内、外分泌胰腺肿瘤的各种细胞和分子生物学特性提供了一个较有价值的体外模型。
The establishment of PC en cell line will provide a useful model in vitro for further investigating the cellular and molecular biology of exocrine and endocrine tumors of the pancreas.
目的：1建立高压体外培养视网膜神经节细胞模型并观察压力对视网膜神经节细胞的影响；
PURPOSE: 1 To build high pressure cell culture model in vitro of Retinal Ganglion Cells(RGCs) and observe the RGCs effected by pressure .
目的建立体外丙型肝炎病毒感染的MT - 2细胞模型。
Objective To establish an MT-2 cell model infected by HCV in vitro.
目的为建立肿瘤细胞向腹膜转移的体外侵袭模型，建立改良的人腹膜间皮细胞消化培养法。
Objective To establish a modified enzymatic disaggregation method to isolate human peritoneal mesothelial cells in order to establish a invasion model of peritoneal metastasis in vitro .
方法：制备贫血小鼠模型，随机分为给药组和对照组，采用造血祖细胞体外培养等实验血液学技术。
Methods:Anemic models of mice were randomly divided into two groups:administered group and control group. Techniques of hematopoietic progenitor cells culture in vitro, etc were used.
通过筛选法测定候选药物在体外，宿主细胞以及动物模型中的抗病毒活性。
Drug candidates are tested for antiviral activity using screening assays in vitro and host cells, as well as in animal models.
方法：建立微载体-基质细胞体外造血模型。
Methods: The hematopoietic model for stromal cell adhering to microcarrier was established.
肝亚细胞成分是研究药物代谢及种属差异的较好体外模型。
The results suggest that hepatic subcellular fractions are useful as in vitro models for species difference study.
采用MTT比色法观察梅花鹿免疫细胞活性因子对环磷酰胺造成体外免疫抑制及小鼠抑制模型的调节效应。
The immunoregulatory effect of DICAF on the in vitro immunosuppression caused by cyclophosphamide and in vivo immunosuppression in mouse model were detected by MTT colorimetric method.
目的：建立一种新型的体外培养成骨细胞的生物学模型。并对其生物学特性进行研究。
Objective: To establish a new model of osteoblasts culture in vitro and study it's biological characterization.
目的：建立人眼小梁细胞体外滤膜培养体系及通透阻力测定的模型。
Purpose: To establish the culture system of human trabecular cells on filter and detect their TEER (trans epithelial electrical resistance).
目的：观察神经生长因子(NGF)诱导PC 12细胞周期的变化，以建立体外神经元样细胞周期的研究模型。
Objective: to observe the effect of nerve growth factor (NGF) on cell cycle in PC12 cells, and to establish a cell cycle model of neuron-like cell in vitro.
它们提供了早期胚胎分化的体外模型，也是基因操作的重要靶细胞。
They provide an in vitro model of early embryonic differentiation and are useful means for targeted manipulation of the genome.
体外肝细胞模型(主要是原代肝细胞培养)作为一种强有力的体外研究体系被广泛应用于化学异物的肝毒性研究。
In vitro liver cell models (mainly primary hepatocyte cultures), served as the most powerful in vitro research system are widely used for the study of hepatotoxicity of xenobiotics.
方法用体外培养的猪血管内皮细胞和不同人的血清共同反应，建立猪与人异种移植超急性排斥反应的体外实验模型。
Methods The in vitro cultured porcine vascular endothelial cells(PVEC) and human sera were co reacted to set up an in vitro model of hyperacute rejection of pig to human xenotransplantation.
方法体外细胞培养，H2O2处理PC 12细胞复制氧化损伤模型。
Method PC12 cell was exposed to H2O2 in vitro to establish oxidative injury model.
方法制备环磷酰胺的体外细胞抑制模型及小鼠抑制模型。
Methods The in vitro (cell) and in vivo (mouse) models of immunosuppression were established.
目的：建立体外研究精原干细胞与支持细胞共培养的细胞模型，探讨体外精原干细胞在支持细胞层上的增殖特点。
Objective: to construct cell model of spermatogonial stem cells (SSCs) with Sertoli cell layer in vitro, and study the proliferation characteristic of SSCs in vitro.
结论：H2O2可以诱导体外培养的人脾细胞凋亡，建立人脾细胞凋亡模型。
CONCLUSION: H2O2 can induce the apoptosis of human spleen cells in vitro, which can be used to establish apoptotic model.
方法利用体外细胞培养模型，应用MTT法分析姜黄素衍生物对不同肿瘤细胞生长的影响。
METHODS Based on cell culture model in vitro, MTT test was used to study the inhibitory effect of curcumin derivatives on tumor cell lines.
方法：利用体外肿瘤细胞血管内游走及血管外游走模型，定量地评价纤维肉瘤细胞(HT1080)的血行转移情况。
Methods The models of intravascular and extravascular migration of tumor cells in vitro were used to observe transvascular metastasis of sarcoma cells (HT1080).
目的：探讨基质金属蛋白酶2 (MMP - 2)在体外构建的C6细胞侵袭模型中的作用。
Objective: To study the function of matrix metalloproteinase-2 (MMP-2) in the selected C6 cells invasion model in vitro.
结论：EBNA 1阳性肿瘤细胞株的建立，为体外进行EB病毒相关肿瘤的研究提供细胞模型。
Conclusions: The EBNA1 positive tumor cell strains may serve as good tools for research in EBV associated tumors.
目的：探讨体外诱导外胚间充质细胞向骨骼肌细胞分化的潜能，利用分化细胞构建组织工程骨骼肌模型。
AIM:To investigate the ability of dimethyl sulphoxide(DMSO) on inducing the ectomesenchymal cells to skeleton muscle cells and using these cell to construct the three-dimension model.
由于HCV体外培养尚未成功，至今仍无满意的细胞和动物感染模型，极大地限制了HCV的研究及丙型肝炎的防治工作。
The progression of prevention and therapy of HCV infection was hampered by lacking reliable cell cultures in vitro and successful small animal models.
目的：在体外建立大鼠耳蜗螺旋神经节神经元培养模型，并提高其产出率及细胞活性。
To establish a cytological model of spiral ganglion neurons in vitro and to improve cell yield and viability.
方法利用体外培养的单层近融合期人RPE细胞，采用棉签和角膜移植用环钻做圆形细胞刮伤区，建立体外RPE细胞损伤模型。
Methods Cultured monolayer-confluent human RPE cells were scraped with a trephine and a cotton stick, and set up the injured model of RPE cells with round scraped area.
方法：以多种复合刺激交替进行的方法建立慢性应激抑郁模型，并取脾脏进行淋巴细胞体外培养。
Methods:Chronic stress of depression model was made by alternate mulriple stimulate, and lymphocyte of spleen was cultured in vitro.
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