建立了一种经济、简便的一步酶法 ，大量合成尿苷二磷酸葡萄糖 。
The authors set up a one-step enzymatic method to synthesize UDPG in much higher yields.
通过选择适宜溶剂，对一步酶法提取阶段所得渣质进行二步溶剂提取，达到充分提取有效成分的目的。
The residue from enzyme-based step can be further extracted in the second solvent-based step by selecting proper solvent in order to extract effective component sufficiently.
方法：采用一步酶法测定血清中MMP -2的表达量，用免疫组化法检测MMP - 2在实体瘤中的表达情况。
Methods: The level of MMP 2 in serum was detected by a step sandwich enzyme immunoassay technique.
目的：通过对酶联免疫吸咐试验(ELISA)双抗原夹心一步法和二步法检测人类免疫缺陷病毒(HIV)抗体的比较，探讨各自特点。
Objective: To compare the characteristics of the one step and two steps ELISA (Enzyme Linked Immunosorbent Assay) diagnostic kit in detecting of human immunodeficiency virus (HIV) antibodies.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
作者认为，应用尿素酶试验同时进行直接涂片法或W -S银染法检查，可进一步提高阳性率。
It is supposed that the rapid urease test in combination with direct smear examination or the W-S silver method could reveal the positive rate more accurately.
化学修饰法研究进一步证明侧链羧基是酶的必需基团，并测定出酶活性必需的羧基数为一个。
These results showed that the ionizing group of the enzyme active center is the carboxyl group.
和化学脱墨相比，酶法提提要：脱墨可以进一步提高纸浆的白度和油墨脱除率，并大幅度降低废水的污染负荷。
Moreover, the deinking effluent from the enzymatic deinking process has lower COD or BOD content than that from the chemical deinking process.
进一步通过荧光光谱法证实，含多拷贝11 -氨基酸基序的多肽可通过多位点协同结合模式，稳定LDH酶的结构。
Furthermore, the fluorescence spectroscopic results show that the peptide consisting of multi-copies of 11-amino acid can stabilize the structure of LDH through synergy and multi-sites binding.
用单抗二抗一步法酶联免疫试验检测感染家兔中的日本血吸虫循环抗原。
A one step dot ELISA using this specific McAb and a 2nd antibody was used for detection of circulating schistosome antigen in the urine of infected rabbits.
用单抗二抗一步法酶联免疫试验检测感染家兔中的日本血吸虫循环抗原。
A one step dot ELISA using this specific McAb and a 2nd antibody was used for detection of circulating schistosome antigen in the urine of infected rabbits.
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