目的:利用寡核苷酸芯片检测SARS病毒。
Objective: To detect SARS virus by hybridization in oligonucleotide array.
利用水稻寡核苷酸芯片检测了去胚水稻种子的基因表达。
RNA profiling using a rice (Oryza sativa cv Nipponbare) oligonucleotide microarray was used to monitor gene expression in de-embryonated rice grains.
方法制备了包含87个心血管疾病相关基因探针的寡核苷酸芯片。
Methods Oligonucleotide microarrays consisting of 87 probes representing 87 human cardiovascular disease-related genes were constructed.
目的设计一种带有通用序列标签的寡核苷酸探针以应用于寡核苷酸芯片点样的质量控制。
Aim To design an universal sequence-tagged oligonucleotide probes for spot quality control of oligonucleotide microarrays.
通过试验,本研究获得了以下成果:(1)制作出背景低,杂交效率高的通用寡核苷酸芯片;
This research attains the following achievements: (1) The universal oligonucleotides microarray has the virtues of low background and high probed binding efficiency;
应用寡核苷酸芯片后,比较1级和2 - 4级溃疡病人中,金黄色葡萄球菌的耐药性和致病基因。
Using oligonucleotide arrays, s. aureus resistance and virulence genes were compared between grade 1 and grade 2-4 ulcers.
目的观察标记引物法、随机渗入标记法、末端转移标记法三种荧光标记法对寡核苷酸芯片杂交信号的影响。
Objective to observe the impact of three fluorescence marker methods of primer marker, random inleakage marker and terminal transfer marker to Oligonucleotide microarray hybridize signal.
结果:SARS阳性样品与寡核苷酸芯片杂交后出现阳性杂交信号,具有不同二级结构的寡核苷酸探针杂交信号强度不一。
Results: Positive SARS samples were detected by oligonucleotide array and fluorescence signal intensity was highly related to the probe's second structure.
其他一些人,比如哈佛大学的GeorgeChurch,采取比较中间的位置,运用寡核苷酸芯片来合成个体基因成分,然后把它们拼在一起。
Others, like George Church of Harvard University, fall somewhere in the middle, synthesizing individual genetic components using oligonucleotide chips, then piecing them together.
目的:建立寡核苷酸膜芯片技术检测SARS病毒。
Objective: To set up an oligonucleotide membrane chip technique for detecting SARS virus.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
依其结构,DNA芯片可分为两种形式,DNA阵列和寡核苷酸微芯片。
According to the structures, DNA chip can be divided into two variants, DNA array and oligonucleotide microchip.
结论采用寡核苷酸阵列芯片技术可对HCV进行基因型以及基因亚型的分析,方法快速、简便、敏感、特异。
Conclusions This method is highly specific, sensitive and rapid for the diagnosis of the genotypes and sub-genotypes of HCV.
结论采用寡核苷酸阵列芯片技术可对HCV进行基因型以及基因亚型的分析,方法快速、简便、敏感、特异。
Conclusions This method is highly specific, sensitive and rapid for the diagnosis of the genotypes and sub-genotypes of HCV.
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