不同菌株原生质体产量差异不显著。
There was also no significant difference in the yield of protoplasts from different strains.
随纤维素酶浓度升高，原生质体产量及活力增大。
Yieldand viability of mesophyll protoplasts were increased as Cellulase concentration was increased.
结果表明，质壁分离的时间、酶液浓度、渗透压及酶解时间是影响原生质体产量和质量的决定性因素。
The results showed that plasmolysis time, concentration of enzyme solutions, different osmoticum and incubation time significantly affected isolation of protoplasts from K326 leaves.
蜗牛酶和1.5％溶壁酶酶解金针菇菌丝获得了高产量的原生质体。
High yield of protoplasts has been obtained by using enzyme containing 1% snailase or 1.5% lywallzyme.
同时，对原生质体再生菌株的形态与多杀菌素产量变化进行了比较研究。
In addition, we compared the change of morphology and spinosyns yield of the regenerated strains.
通过对A4原生质体紫外线诱变筛选得到酒精产量明显提高的酵母菌株。
The yeast increased the ethanol production was screened by UV-mutation of A4 protoplast.
阿氏假囊酵母在原生质体再生过程中具有较高的突变率，以维生素B＿2产量为指标的正突变率为12．14％。
Many mutants appeared in the regeneration strains on solid medium. Positive mutation ratio was 12.14% by determining the yield of Vitamin B_2.
结果表明：枣叶片分离出的原生质体活性显著高于悬浮培养物，但是产量低于悬浮培养物分离出的原生质体。
The results showed that the viability of protoplasts isolated from leaves was significantly higher than that of the suspension culture, but the yield of protoplasts was just the opposite.
在酶解之前对材料进行质壁分离处理使原生质体的产量提高了3.23％，存活率提高了4.2％。
Moreover, if the young leaf pieces were plasmolyzed before incubated in enzyme solution will increased the yield and activity of protoplast 3.23% and 4.2%.
为提高海南霉素产量，分别对其产生菌进行氮离子束注入、原生质体再生等处理，获得高产菌株2-7、Y81。
High producing mutants 2 7 and Y81 of hainanmycin were obtained by ion beam implantation or protoplast regeneration.
渗透压调节剂的浓度为0.5M，以蔗糖作为渗透压调节剂优于甘露醇和葡萄糖，但是此时原生质体的产量下降28.7—38.1％；
Osmotic medium concentration is 0.5M. The sugar is superior to mannitol and glucose. However, in the osmotic medium the yield is decreased within 28.7-38.1%.
渗透压调节剂的浓度为0.5M，以蔗糖作为渗透压调节剂优于甘露醇和葡萄糖，但是此时原生质体的产量下降28.7—38.1％；
Osmotic medium concentration is 0.5M. The sugar is superior to mannitol and glucose. However, in the osmotic medium the yield is decreased within 28.7-38.1%.
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