ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
目的探讨体外原代培养大鼠肝细胞的分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Conclusions: Hyperthermia may induce apoptosis of neuroepithelium in primary culture.
结论：高温可诱发原代培养神经细胞凋亡。
Some factors affecting the primary culture of Penaeus chinensis lymphatic cells were studied.
以中国对虾为实验材料，研究影响对虾淋巴组织原代培养的一些因素。
Objective To establish a method for the primary culture of bovine retinal capillary pericytes.
目的：建立牛视网膜毛细血管周细胞原代培养的方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
目的探索人心房肌细胞的原代及传代培养方法。
ConclusionAdding lens cortex can increase the success rate of primary culture of lens epithelial cells.
结论添加晶体皮质可提高晶体上皮细胞原代培养成功率。
A primary culture of myoblasts may be prepared by excising skeletal muscle tissue from 10 to 30 day old rat embryos.
制备成肌细胞的初级培养物，可以将10-30天的大鼠胚胎骨骼肌组织剪切下来。
Results the area positive for ALP staining is no less than 90% in the primary culture and almost 100% in the subculture.
结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。
Objective: to establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tis-sure.
目的：建立一种简单、较理想的新生大鼠皮层神经元体外原代培养方法。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Objective To observe the changes of limbal stem cell morphology? Antigen and proliferative ability after primary culture in vitro.
目的探讨人角膜缘干细胞体外培养后细胞形态学、抗原性和增殖能力的变化。
HLF cells in primary culture shows fibroblast-like phenotype. Biological characteristics of cells within 5 generations are stable.
体外培养的黄韧带细胞呈成纤维细胞样表型，细胞在传5代以内生物学特性稳定。
MethodsA primary culture of rabbit corneal fibroblast was established to observe the effect of MMC and HA on cellular proliferation.
方法对兔角膜成纤维细胞体外培养，观察MMC及HA对细胞生长的影响。
Objective: to explore the primary culture method of human chorionic and decidual tissues in vitro and to study their growth regular.
前言：目的：探讨体外培养人绒毛膜组织和蜕膜组织的方法，并对其体外生长规律进行研究。
Objective: To establish a convenient and efficient method of primary culture of rabbit brain microvessel endothelial cells(BMVEC) in vitro.
前言： 目的：建立简便、有效的兔脑微血管内皮细胞体外原代培养方法。
Objective: to establish a human hepatocellular carcinoma cell line EHH1 by primary culture and to investigate its biological characteristics.
目的：采用原代培养的方法建立一株人肝癌细胞系ehh1，并对其生物学特性进行分析。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
AIM: to compare the primary culture methods of human pulp cells, to enhance the culture condition, and give rise of the success rate of cultivation.
前言：目的：比较不同的人牙髓细胞原代培养方法，优化培养条件，提高培养成功率。
Objective to investigate the apoptosis rate of neurons in vitro among the primary culture cells isolated from rat spinal cords before and after injury.
目的研究体外培养脊髓神经元损伤前后的凋亡变化，进一步探讨中枢神经损伤的分子机制。
Methods The human embryo cerebral neurons were prepared for the primary culture using light microscope, tissue staining after inocula-ting HCMV of TCID50.
方法采用人胚脑神经细胞原代培养，接种TCID50的HCMV后用光镜、组织染色观察病变全过程。
The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.
移植后肿瘤细胞在形态、生长、分泌CA12 5功能方面均与原细胞株保持一致。
Primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment of adding different hormones.
筛选外植体后，再添加不同种类及浓度的生长调节剂对黑水缬草进行愈伤组织诱导和增殖培养、芽诱导和增殖培养及生根培养。
Objective: To establish an easy and high-yield method of rat dopaminergic neuron primary culture, and to observe the damage to the neurites by the trypsogen.
目的：建立一种简单高效的中脑多巴胺神经元细胞原代培养方法，并观察胰酶消化对中脑多巴胺能神经元突起生长的损伤作用。
Conclusion Osteoblasts achieved by this method are pure with large quantities, so this method can be used as a reliable and efficient way of primary culture.
结论酶交替消化法原代培养胎鼠成骨细胞，获得的成骨细胞纯度高、数量大，可作为一种相对可靠、有效的原代成骨细胞培养方法。
So those are the four core models within the Schneider model. It says that typically in the company, there's a primary culture and then a secondary supporting culture.
因此，Schneider模型有四种核心模型，它认为：公司中一般都有一种基本文化，然后还有一种支持性文化。
NECL2 distributed in cell body and protuberance of primary culture neuron. The rat adrenal gland tumor cell line PC12 can be induced into neuronal differentiation.
大鼠肾上腺嗜铬细胞瘤细胞PC12具有神经元样分化的潜能，在PC12细胞中转染并过量表达NECL2能够诱导细胞发生神经元样分化。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
方法：(1)建立与改良小鼠成骨细胞的原代培养方法，并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
方法：(1)建立与改良小鼠成骨细胞的原代培养方法，并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
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