Purpose To establish a method for culturing rabbit limbal stem cell in vitro and observe its biological characteristics.
目的建立兔角膜缘干细胞体外培养方法，观察其生物学特性。
Objective to study the treatment of rabbit limbal stem cell deficiency by cultured limbal stem cells growing on amniotic membrane for autograft transplantation.
目的观察以羊膜为载体的兔角膜缘干细胞膜片移植治疗兔角膜缘干细胞缺损的效果。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Conclusion: the method treating the rabbit ocular surface alkali burns with corneal limbal stem cell allograft is an effective therapy.
结论：含干细胞的异体角膜缘移植治疗眼表碱烧伤的疗效明显优于单纯异体角膜移植，是一种较有效的方法。
Conclusion: the method treating the rabbit ocular surface alkali burns with corneal limbal stem cell allograft is an effective therapy.
结论：含干细胞的异体角膜缘移植治疗眼表碱烧伤的疗效明显优于单纯异体角膜移植，是一种较有效的方法。
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