We're going to use that Ampicillin resistance in technological ways and I'll describe that in a minute.

我们将通过技术手段,利用这种氨苄青霉素抗性,马上我就会讲到

耶鲁公开课 - 生物医学工程探索课程节选

If this plate has Ampicillin in it, then the only cells that would be able to grow here are cells that have resistance to Ampicillin.

如果培养基上有氨苄青霉素,那么能在这里生长的,只有那些对氨苄青霉素有抗性的细胞

耶鲁公开课 - 生物医学工程探索课程节选

This process of using a biological event like resistance to Ampicillin in order to pick out the cell population that you're interested in is called selection.

这个利用生物特性,如对氨苄青霉素的抗性,来挑出你所感兴趣的细胞的过程叫筛选

耶鲁公开课 - 生物医学工程探索课程节选

If a cell has a gene that makes it resistant to Ampicillin, that means that that micro-organism can survive being exposed to this normally deadly chemical without dying.

如果细胞带有一种基因,能够让它对氨苄青霉素产生抗性,即使把它放在,有致死剂量化学物质的环境中,它也能存活下来

耶鲁公开课 - 生物医学工程探索课程节选

If you selected the cells right than the only ones that have that resistance to Ampicillin are the ones that successfully got your plasmid and are using this Ampicillin resistant gene.

如果你选对了细胞,那么对氨苄青霉素有抗性的就是,成功得到了质粒并表达了基因的细胞

耶鲁公开课 - 生物医学工程探索课程节选

You could imagine strategies where you have multiple resistance genes on a plasmid, resistance to Ampicillin, to Penicillin, to Erythromycin for example, and you design strategies for separating out which cells are carrying the plasmid that you're interested in.

若质粒上有多种抗性基因,你就有很多筛选方法,例如对氨苄青霉素,青霉素,红霉素的抗性,而且你可以设计方法分离出,含有你感兴趣质粒的细胞

耶鲁公开课 - 生物医学工程探索课程节选

If we grew these cells on a plate loaded with Ampicillin and we could select cells that have Ampicillin resistance, and this process of selection and cell culture is very important and we'll talk about it more next week.

如果我们在含有氨苄青霉素的,培养基上培养细胞,我们可以筛选出,对氨苄青霉素有抗性的细胞,这个筛选和培养细胞的过程是很重要的,我们下星期会讲到这些

耶鲁公开课 - 生物医学工程探索课程节选

The property it gives here is called AMPR or resistance to Ampicillin.

这里给出的这种特性叫做AMPR,即对氨苄青霉素的抗性

耶鲁公开课 - 生物医学工程探索课程节选