They do that in a very special way in that they - restriction enzymes are able to identify a particular sequence of bases in a gene.

限制性内切酶的工作机制很特别,它能识别基因中的一段特定的碱基序列

耶鲁公开课 - 生物医学工程探索课程节选

It takes advantage of the specificity of the restriction enzymes, the fact that we know what the gene sequence that we're looking for, and using this technological process of electrophoresis to identify changes that we predict.

这个方法利用了限制酶特有的性质,我们知道我们在找什么样的基因序列,从而才能利用电泳这个技术流程,来识别出我们预计的情况

耶鲁公开课 - 生物医学工程探索课程节选

If this was a sickle patient, so they had this gene instead it wouldn't get cut and when I went to look for that presence of that gene on this gel, it would appear as one large segment instead of a large one and a smaller one.

而如果是个镰状红细胞贫血的病人,他们的基因是不会被限制酶切割的,因此他们基因的凝胶电泳结果就不会呈现,一大一小的结果,而只有一个大的片段

耶鲁公开课 - 生物医学工程探索课程节选

In a normal cell DNA that DNA in the normal cell - this DNA gets cut by the restriction enzyme, so the sickle gene ends up - so that hemoglobin gene ends up in two pieces.

一个正常细胞的DNA,正常细胞里的DNA,会被限制酶所剪切,因此镰状血红蛋白基因--,不对,是正常血红蛋白基因被切成两段

耶鲁公开课 - 生物医学工程探索课程节选