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The pasting back together occurs partly naturally by this process of hybridization, but hybridization only re-establishes the base pairing.
片段和载体的重新粘合一定程度上,是通过杂交自然发生的,但杂交只能重建碱基配对
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We talked about base pairing and how that leads to this process of hybridization or very specific matching between complimentary strands.
我们讲了碱基配对,以及它们如何引起杂交过程,或者说,互补链之间的特异配对
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The correct complement is made because of these principles of Watson-Crick base pairing that we talked about before.
剪接配对的正确性源自,沃森克里克碱基互补配对原则的作用,这我们讲过的
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These will naturally want to reform and they'll want to reform to re-establish this base pairing.
它们会自然地重新进行碱基配对
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I just give you one of those, right, because you could write down the other one because you know the other complimentary sequence from base pairing?
我只写了一条链的序列,因为你们能够写出另一条,因为你们根据碱基配对,就能推测出互补链的序列
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If you do the base pairing goes exactly the same sequence backwards down here.
如果将它碱基配对,就会得到相同,但反过来的序列
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Translation occurs by a special kind of polymerization where these transfer RNA's operate by Watson-Crick base pairing.
翻译过程是一种特殊的聚合过程,当tRNA按照,沃森克里克碱基互补配对原则进行配对
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It exists in a double stranded form where every strand is matched by its complement.
这种碱基配对存在于DNA双链中,每条单链都与其互补链,通过这个原则配对组合
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It has to do with the nature of this complementary binding between double stranded DNA and the fidelity of this base pair matching in forming stable DNA molecules.
这些特性能从根本上解释,为何DNA双链的碱基能互补配对,以及在形成稳定的DNA分子结构时,碱基互补配对为何具有一致性
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If I'm talking about two DNA strands and they differ only in one or two base pairs they won't be exact complements and they won't form this double helix.
假设有两条DNA单链,有一两个碱基不能配对,它们就不能完全互补,也无法形成双螺旋形结构
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Well you do that by this Watson-Crick base pairing, so I know if I have I know what messenger RNA to make from that because I have to satisfy these base pairing rules.
依据的是沃森克里克碱基互补配对原则,如果给我一个ACGCGA的序列,就能知道转录出的mRNA会是什么,因为我必须满足碱基配对原则
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The native structure is this double stranded structure here and if I heat it up slightly and I add some base, so under slightly basic conditions, these molecules will fall apart because you've created conditions where the hydrogen bonding is no longer favorable so they peel apart.
原始结构,在这里就是指DNA双链,如果我稍微加热,并且加入一些碱,在弱碱性的条件下,这些配对碱基会分开,这是因为在一定条件下,碱基对之间的氢键,不再稳定并且会断裂分开
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