You first separated your DNA, let the primers bind, then turn on the enzyme, and it makes copies of each one of these.

首先解旋DNA双链,使引物结合,接着激活聚合酶,最终得到各链的复制产物

耶鲁公开课 - 生物医学工程探索课程节选

The polymerase starts a process of replication of your DNA sequences.

聚合酶使DNA序列开始复制

耶鲁公开课 - 生物医学工程探索课程节选

Remember we talked about how DNA replicates itself and that there are enzymes, DNA polymerase that bind to the double stranded DNA, separate it, denature it locally, and then start the process of replication.

请记住DNA复制必须有酶的参与,DNA聚合酶结合在双链DNA上,打开双链并原位变性,然后才开始复制

耶鲁公开课 - 生物医学工程探索课程节选

A primer is a short RNA sequence or DNA sequence that gets sort of the process of replication jump started, and that's just because of the biological properties of DNA polymerase that that primer's needed.

引物是一段短的RNA或者DNA序列,引物是整个复制的起点,因为DNA聚合酶的,生物特性,引物在复制过程是必不可少的

耶鲁公开课 - 生物医学工程探索课程节选

That DNA gets replicated to make many more copies of the viral DNA using host mechanisms, that is, often using the DNA polymerase which is naturally present in the host cell for its own replication.

病毒DNA进行复制,通常是利用在宿主细胞自身的有关物质,利用宿主细胞中天然存在的,DNA聚合酶来大量复制病毒DNA

耶鲁公开课 - 生物医学工程探索课程节选

Synthesis always occurs in one direction and that makes sense to you now because you know there's a directionality and the chemistry is different going one way than the other and this DNA polymerase only works on the chemistry going in one direction.

NA复制过程是单向的,现在你们该明白原因了,因为DNA单链有方向性,DNA在两个方向上化学性质不同,DNA聚合酶,只在一个方向上起作用

耶鲁公开课 - 生物医学工程探索课程节选