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Get a gene, put it in a plasmid, get that plasmid expressed in a host, could even be a bacterial host,right?
把一个基因导入一个质粒,让质粒在宿主中表达,这个宿主甚至可以是细菌,对吧
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Or, if you are at a party and you're ready to leave but you've left your bag with the host,
又或者，你在一个派对上，你准备要走了，但是你刚才把你的包放在派对主人那里了，
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Well, it turns out that we could not identify, at least at that point, a cell culture that would serve as a host for vaccinia.
然而我们还不能断定,至少这个时候无法断定,这些被感染的细胞就是牛痘病毒的宿主
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It seemed I had a whole host of memories going back to 1750 in England and that I was in San Francisco now only in another life and in another body.
似乎全部记忆都回到了1750年的英格兰,而现在在圣弗兰西斯科的我则是另一个人，在另一种生活里。
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God is depicted as a warrior, who leads his host , the Lord of hosts in battle.
上帝被描述为武士，他带领军队战斗，他是战争中军队之神。
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And sexual selections give rise to a host of organisms, some of which survive and are fit and others which aren't.
性选择导致了大量有机体的产生，其中的一些有机体生存了下来,适应了竞争,而其他则遭到淘汰。
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Fortunes will begin to be made in the textile industry and in the railroad industry by the '40s and '50s, and in a host of other ways, real fortunes.
在40 50年代的时候,纺织业和铁路工业将会制造大量的财富,另一些方面,真正的机遇
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By extending your time horizon to three years, or four years,or five years, it opens up a whole host of investment opportunities that aren't available to people that are playing this silly,short-term game.
只有通过将投资延长至三年,四年乃至五年,才会出现大量的获利机会,沉迷于愚蠢的短线操作,是无法得到这些机会的
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Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.
取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制
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This connects I think with a whole host of other passages that have to do with nothingness.
我认为这和文章,关于虚无的其他部分有联系。
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They integrate their DNA into the host genome and you might not want to make that kind of a change in the cell.
而且也许你也并不想让逆转录病毒基因,整合进宿主的基因组
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Otherwise you could have a squirrel host-- I'm using squirrels, because I think that's funnier than other animals to think of— but it could be any potential host, right?
否则松鼠就会变成宿主,我拿松鼠举例,是因为我觉得它比别的动物更有趣,但潜在宿主可以是任何动物,对吧
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Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.
将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达
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Now, for those of you who don't know, On the Road does document pretty closely the actual road trips that Jack Kerouac took with Neal Cassady and a whole host of others, and I can do a little decoding for you.
现在，如果你们中有人不知道，《在路上》确实近乎于是对真实中,杰克凯鲁雅克带着尼尔卡萨迪和其他一群人上路旅行的记载，我来给你们做些破译。
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Well, if you knew a cell that could serve as a host for a virus, you could use this process of cell culture to make large quantities of virus,right?
如果你知道细胞可以变成病毒的宿主,可以用培养细胞的过程,制造大量病毒,对吧
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Some words that are useful in this discussion, a 'host'. A host is the organism that you're interested in defending and it could be you or me or some typical person.
讨论中会用到一些词,宿主 宿主是,你对其免疫反应感兴趣的生物体,可以是你我,或者其他人
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