They tested the effects of calorie restriction in seventy-six rhesus monkeys over twenty years.

VOA: special.2009.09.01

That's an example of symmetric sequence and it happens that most restriction enzymes also recognize those spaces.

这是一个对称序列的例子,大多数限制性内切酶都能识别这些序列

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So that's often a big restriction that a lot of dorms will put. But here, here at law school,

这是许多宿舍明令禁止的。但是在法学院，

关于宿舍制度 - SpeakingMax英语口语达人

Richard Weindruch explains. "Our data show that there is about a three-fold higher risk of developing a disease of aging in those animals fed the normal diet as opposed to those that have been on caloric restriction since they were adults."

VOA: special.2009.09.01

They do that in a very special way in that they - restriction enzymes are able to identify a particular sequence of bases in a gene.

限制性内切酶的工作机制很特别,它能识别基因中的一段特定的碱基序列

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The loss of jobs and business opportunities has perhaps been more damaging than the restriction on imports, especially for those who under the blockade - cannot cross into Israel.

VOA: standard.2010.06.16

This takes advantage of the very specific properties of restriction enzymes.

这利用了限制酶的独特性质

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It takes advantage of the specificity of the restriction enzymes, the fact that we know what the gene sequence that we're looking for, and using this technological process of electrophoresis to identify changes that we predict.

这个方法利用了限制酶特有的性质,我们知道我们在找什么样的基因序列,从而才能利用电泳这个技术流程,来识别出我们预计的情况

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When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

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What are the properties of this restriction enzymes?

这种限制性内切酶的特性是什么

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There's whole families of restriction enzymes.

限制性内切酶是一个大家族

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Now another property of restriction enzymes is that they always cut the DNA in the same way.

限制性内切酶的另一个特性是,总是以同样的方式切割DNA

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Most restriction enzyme also recognize symmetric sequences of DNA, GAATTC for example.

大多数限制性内切酶,也能识别DNA的对称序列,例如GAATTC

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Restriction enzymes are just a kind of enzyme, enzymes are protein molecules that make a chemical reaction go faster, and the chemical reaction that restriction enzymes do is cutting DNA.

限制性内切酶是一种酶,而酶是加速化学反应的蛋白质分子,限制性内切酶加速的化学反应,是切割DNA

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If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

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If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.

而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对

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Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.

如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交

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