One of the things about these plasmids that makes them very useful is that their entire base pair sequences is known.

质粒之所以有用,其中一个原因就是,它的全部碱基序列都是已知的

耶鲁公开课 - 生物医学工程探索课程节选

It has to do with the nature of this complementary binding between double stranded DNA and the fidelity of this base pair matching in forming stable DNA molecules.

这些特性能从根本上解释,为何DNA双链的碱基能互补配对,以及在形成稳定的DNA分子结构时,碱基互补配对为何具有一致性

耶鲁公开课 - 生物医学工程探索课程节选

You know that messenger RNA is read in three base units called codons, and so this particular piece of messenger RNA is drawn in this cartoon in three base pair units.

RNA是按每三个碱基组成一个密码子,进行翻译的,在这幅动画中所画的mRNA,就是按三个碱基一组的方式来描绘的

耶鲁公开课 - 生物医学工程探索课程节选

That's because you have to satisfy this base pair matching in order to have hydrogen bonding in each of the struts of the ladder in order to form a stable structure.

NA双链要满足互补配对原则,以使碱基对之间,形成氢键,从而形成稳定的双链结构

耶鲁公开课 - 生物医学工程探索课程节选

This label is DNA that might be made radioactive or made fluorescent, and it has a base pair sequence that is from some other region of the gene that you're interested in.

这种标签就是具有放射性,或者荧光性的DNA,它有一个取自于,你感兴趣的那段基因上,其他位置的碱基对序列

耶鲁公开课 - 生物医学工程探索课程节选

Well one way you could do it is by saying 'if I have this one base pair difference then this sequence is going to be cut by a specific restriction enzyme that recognizes the sequence CTGAGGA'.

一种方法是通过考虑,"如果我有这个不同的碱基对,那该序列必然会,被特定的限制酶识别并切割,这个特定的限制酶能识别,CTGAGGA "

耶鲁公开课 - 生物医学工程探索课程节选

Every cell in your body has exactly the same DNA; that is if I could stretch out all the DNA and look at the base pair sequence, the sequences of bases along all the DNA in your chromosomes, they'd be identical in all the cells.

身体内每一个细胞都有完全相同的DNA,如果我把整条DNA展开,然后检查其碱基对的顺序,你体内每个细胞中染色体上,DNA的碱基对顺序,都是完全相同的

耶鲁公开课 - 生物医学工程探索课程节选