Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体,并在COS - 7细胞中表达。
Objective To construct a recombinant plasmid containing rhoptry protein 2 (ROP2) gene of Toxoplasma gondii .
目的构建弓形虫棒状体蛋白2 (ROP2 )基因重组质粒。
To construct a recombinant plasmid containing protein transduction domain (PTD) and brain derived neurotrophic factor (BDNF) fusion gene and express in e.
构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒,并在大肠肝菌中表达。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌,获得纯化的P30蛋白抗原表位,用于检测弓形虫特异性抗体。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的:构建弓形虫棒状体蛋白(ROP2 )基因重组质粒并在大肠杆菌中表达,用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的:构建高效表达人透明带蛋白3的真核重组表达载体。方法:利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的:构建高效表达人透明带蛋白3的真核重组表达载体。方法:利用PCR、T- A载体克隆和亚克隆等技术。
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