METHOD Using whole cell patch clamp technique.
方法采用全细胞膜片钳制技术。
METHOD Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳技术。
Methods Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳制技术。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
Whole cell patch clamp technique was used to record the action potential and ion currents.
以全细胞膜片钳记录单细胞跨膜动作电位和离子电流。
Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs.
方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
The characterizations of voltage-activated potassium channels were studied on newborn bovine retinal neurons in long-term cultures with whole-cell patch clamp technique.
目的探讨体外培养不同时期新生小牛视网膜神经元电压门控钾离子通道的特性。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
The patch clamp technique has become the main method for cell electrophysiology research.
膜片钳技术已成为细胞电生理学研究的重要手段。
The patch clamp technique has become the main method for the molecule and cell research.
膜片钳技术已成为分子和细胞学研究的重要手段。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods: Patch clamp whole cell recording technique was used.
方法：膜片钳全细胞电流记录方法。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.
以原代培养的大鼠海马神经元为标本，运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。
This article reviewed lately research status of Chinese herbal effect on calcium channels of myocardial cell by patch clamp technique and look ahead the future research.
本文综述了近年来利用膜片钳技术研究中药对心肌细胞膜钙通道作用的现状，并对未来研究进行展望。
METHODS Using cell-attached patch clamp technique.
方法采用细胞贴附式膜片钳技术。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
The sodium currents were detected by patch-clamp technique in whole-cell recording mode.
用膜片钳技术的全细胞模式测定全细胞钠电流并观察应用特布他林对它的影响。
METHODS: The date recording from cell - attached patches of patch - clamp technique.
方法：采用细胞贴附式的膜片钳技术。
Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.
方法：通过膜片钳手段，测量未分化的SHSY5Y细胞钙离子通道电流；
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
METHODS: Cell-attached configuration of patch clamp technique.
方法：细胞贴附式膜片钳技术。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术，我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流（NSCC）。
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