目前，很多皮肤研究员，包括宝洁公司的皮肤研究员，已经开始使用制药业中常用的DNA微序列来分析不同年龄肌肤中上千基因的表达式。
Now skin researchers, including those at P&G, are starting to use DNA microarrays, common in the drugs industry, to measure the expression of thousands of genes in skin of different ages.
互补；克隆分子；序列分析；基因表达。
DNA, complementary; cloning, molecular; sequence analysis; gene expression.
表达序列标签）测序分析技术广泛应用于基因功能和表达模式的分析研究。
The technology of expressed sequence tags (ESTs) was broadly applied in analysis of gene function and expression pattern.
在现代分子生物学技术研究中，常常需要对已知位点的侧翼序列进行分析或克隆，以研究基因的遗传表达调控。
In modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
以高通量基因组序列(HTGS)数据库及SAGE文库为基础，进行染色体定位及组织表达分析。
Bioinformatic data of High Throughout Genomic Sequences (HTGS) and Serial analysis of Gene expression (SAGE) were used for analysis of chromosome localization and tissue expression.
同时，结合基因表达序列标签进行基因表达分析，对于发现组织特异表达基因具有重要指导作用。
In addition, expression sequence tag data suggests a new way for finding tissue specific expression genes.
方法：应用生物信息学分析软件分析SARS冠状病毒S蛋白的抗原表位序列，设计全新的抗原表位编码基因序列，构建表达载体并在大肠杆菌中表达该基因。
Methods: By analyzing the antigen epitope of SARS-CoV s protein with antigenic peptides prediction software, a new gene was designed, and its expression vector was constructed.
因此选择对基因的表达具有重要功能的外显子1和其上游的调控区及下游外显子内含子交界区序列通过直接测序法进行SNP筛查，并进一步分析多态位点与病窦的关联性。
Therefore the other aim of our study is to discover SNPs of HCN4 exon 1, including the regulatory sequence and exon-intron boundary of it, which plays an importable role of channel function.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
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