Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
But t is not known to redeclare clone as public, so cloning is also out.
但是重新声明clone为public并不知道T，因此克隆也无济于事。
Conclusion it is a general and effective method to clone PCR products with T-vectors.
结论t -载体法是克隆pcr产物通用而有效的方法。
However, the correlation coefficient of clone S11 K content in leaf and K content in soil hasn′t the remarkable relation.
无性系S11叶片K营养元素含量与土壤中K营养元素的回归相关系数不存在显著回归相关。
Objective To clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
目的克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective to explore the feasibility that the recipient against donor antigen specific t lymphocytes clones are formed, and the suicide genes are induced into the clone.
目的探索建立受体针对供体抗原特异性T淋巴细胞克隆，转入自杀基因诱导移植耐受的可行性。
Objective to explore the feasibility that the recipient against donor antigen specific t lymphocytes clones are formed, and the suicide genes are induced into the clone.
目的探索建立受体针对供体抗原特异性T淋巴细胞克隆，转入自杀基因诱导移植耐受的可行性。
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