Selemum with given concentrations into cell cultures could effectively reduce the damages caused by serum free culture.
细胞培养液中加入一定浓度硒可有效地减轻无血清培养引起的细胞损伤。
Objective: to investigate whether CD3McAb activated killer (CD3AK) cells could induce apoptosis in HL 60 cells in serum free culture system.
目的：采用无血清培养体系，研究CD 3单克隆抗体激活的杀伤(CD3AK)细胞诱导HL6 0细胞凋亡情况。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
受精卵在含血清FBS或OCS培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cell culture of serum free medium.
方法：采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元，观察氦氖激光照射对神经元生长发育的影响。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods:Culture CHO cells in serum-free media and then compare the maintainance time, morphological changes and hemagglutination titers of the cells in culture supernatant.
方法：应用无血清培养基采用小方瓶培养CHO细胞，观察细胞维持时间、培养过程的形态变化。
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞；
Objective: to investigate the possible protective effects and related mechanisms of olanzapine on PC12 cells, apoptosis induced by serum-free culture was observed.
目的：探讨奥氮平对无血清诱导PC 12细胞凋亡的保护作用及其机制。
Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.
结论目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。
Conclusion Serum-free and feeder layer-free culture seems to be an ideal method for the cultivation of HKCs.
结论无血清、无滋养层培养，是一种较理想的培养HKC的方法。
Methods kidney cortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶-EDTA消化传代。
C6 (r) technology lie in its excellent safety profile, scalability and productivity under serum-free culture conditions.
C6 (R)技术在于它卓越的安全性，可扩展性和生产力下无血清培养条件。
The present invention provides a serum free medium used for cultivating CHO cellular extensively, and discloses the prescription of the culture medium.
本发明提供了一种用于大规模培养CHO细胞的无血清培养基。
Methods: We selected the best medium fitted for es cells culture, and analyzed the neural differentiation of es cells by comparing increased number of viable cells among different serum-free medium.
方法：比较ES细胞在不同培养基中的生长情况，分析ES细胞在不同时间分化形成神经细胞的比例。
Serum-free culture of mouse neuroblastoma cessl were used for the study of neuronal aging, with microfluorometry of the autofluorescence of lipofuscin as the index of neuronal aging.
采用小鼠神经母细胞癌细胞无血清培养建立神经细胞老化实验研究模型。
Conclusions With the application of the "m" serum - free medium, the technique of Schwann cell culture was simplified and the contamination of fibroblasts was effectively controlled.
结论应用“M”无血清培养液简化了雪旺细胞的培养技术，有效地控制了成纤维细胞的污染。
Instect cell culture media were optimized carefully to find new medium for special cell strain and serum free media was manufactured commercially for suspension cell mass production;
通用的昆虫细胞培养基配方的不断优化，特定昆虫细胞株培养基的研制和适用于大规模悬浮培养的无血清培养基的商品化；
The cell capsule antigen prepared with serum-free culture has good immunity for poultry.
以此制备的禽霍乱荚膜亚单位苗对家禽具有良好的免疫原性。
Methods:1. Hippocampal neurons of neonatal SD rats were isolated and cultured in the serum-free culture media.
方法：1。采用无血清培养法培养大鼠海马神经元，经免疫荧光法进行纯度鉴定。
Methods:1. Hippocampal neurons of neonatal SD rats were isolated and cultured in the serum-free culture media.
方法：1。采用无血清培养法培养大鼠海马神经元，经免疫荧光法进行纯度鉴定。
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