Various reverse transcriptase–polymerase chain reaction (RT–PCR) methods are available but are of variable sensitivity.
有各种逆转录酶聚合酶链反应(扩增核糖核酸基因组RT–PCR)检测试验方法，但灵敏度各不相同。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用逆转录聚合酶链反应(RT - PCR)检测84例静脉毒瘾者血浆标本。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Similar results were obtained through detecting the expression of CXCR4 with reverse transcription-polymerase chain reaction.
反转录-聚合酶链反应法检测CXCR4的表达，亦得到相似结果。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Reverse transcription polymerase chain reaction technique for the detection of CTV in a single aphid or aphids has been developed.
检测橘蚜携带CTV的分子生物学反转录-聚合酶链式反应技术已建立，并已应用于检测橘蚜等蚜虫的单虫带毒情况。
A double - round reverse transcriptase - coupled polymerase chain reaction (RT - PCR) was applied to detect the CYP1A1 transcript.
双轮回‘聚合酶链式反应’(PCR)和‘反向转录酶-聚合酶链式反应’(RT - PCR)被用来检测基因CYP1A1的转录水平。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
40 blind samples were examined by AGAR gel precipitation (AGP), hemagglutination inhibitory (hi) test and reverse transcription-polymerase chain reaction (RT-PCR).
对40份盲样采用琼脂扩散试验(agp)、血凝抑制试验(HI)和反转录聚合酶链反应(RT - PCR)进行了检测。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
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