Various reverse transcriptase–polymerase chain reaction (RT–PCR) methods are available but are of variable sensitivity.
有各种逆转录酶聚合酶链反应(扩增核糖核酸基因组RT–PCR)检测试验方法，但灵敏度各不相同。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
Methods differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.
方法采用差异显示反转录- P CR (DDRT - PCR)方法，寻找骨组织内与健骨冲剂疗效相关的有差异表达的基因。
A double - round reverse transcriptase - coupled polymerase chain reaction (RT - PCR) was applied to detect the CYP1A1 transcript.
双轮回‘聚合酶链式反应’(PCR)和‘反向转录酶-聚合酶链式反应’(RT - PCR)被用来检测基因CYP1A1的转录水平。
40 blind samples were examined by AGAR gel precipitation (AGP), hemagglutination inhibitory (hi) test and reverse transcription-polymerase chain reaction (RT-PCR).
对40份盲样采用琼脂扩散试验(agp)、血凝抑制试验(HI)和反转录聚合酶链反应(RT - PCR)进行了检测。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%.
膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.49%，特异度为100%。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用逆转录聚合酶链反应(RT - PCR)检测84例静脉毒瘾者血浆标本。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Objective To develop a molecular biologic technique for detection of bacterial DNA in all bacteria with PCR and reverse hybridization.
目的探讨聚合酶链反应(pcr)加反相杂交技术在细菌DNA检测中的应用。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Methods The fecal specimens from children with acute nonbacterial gastroenteritis were collected and HuCV in the samples were detected by reverse transcription (RT)-PCR.
方法收集济南市儿童医院婴幼儿病毒性腹泻粪便标本，使用杯状病毒特异性引物对标本进行RT-PCR检测。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
This research will helpful for further study of NDV reverse-genetic system, and the optimization of long RT-PCR will helpful for simplify the construction of clones.
本研究为建立NDV的反向遗传操作系统奠定了基础，同时对长链RT-PCR法的条件优化和简化克隆的构建提供了参考。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
The influence of the Mooney viscosity fluctuation of sulfur-modified powdered CR by reverse-coagulation cover method (PCR-121) on the physical properties of its vulcanizate was investigated.
考察逆凝聚包覆法制备的硫黄调节型粉末氯丁橡胶(PCR12 1)的门尼粘度波动对其硫化胶物理性能的影响。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
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