Objective To investigate the effect of resistin and resistin binding peptide(RBP) on the glucose uptake in L6 rat skeletal muscle cells.
目的探讨抵抗素及其结合多肽（RBP）对大鼠骨骼肌细胞糖摄取功能的影响。
Methods: 1. Primary rat skeletal muscle satellite cell was cultivated in vitro. The cultured cells were identified with cellular immunochemical stain.
方法：1体外分离培养大鼠骨骼肌卫星细胞，免疫组化鉴定骨骼肌卫星细胞并检测其纯度。
Results: Thedielectric response of the skeletal muscle cells from normal rat showed dependence of frequency, discrepancy in orientation, and two characteristic frequencies.
结果：正常大鼠骨骼肌细胞介电响应具有频率依存性，存在方向性差异，其介电弛豫现象表现为两个特征频率。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
取大鼠肱三头肌，采用二步消化法分离骨骼肌卫星细胞，体外进行原代培养和传代培养。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
取大鼠肱三头肌，采用二步消化法分离骨骼肌卫星细胞，体外进行原代培养和传代培养。
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