Objective: To investigate Wenyang Huoxue recipe that could induce the expression of fibronectin (FN) and laminin (LN) by rat renal fibroblasts in vitro culture.
目的:探讨纤维粘连蛋白(FN)、层粘连蛋白(LN)在体外鼠肾成纤维细胞的表达及温阳活血方的干预作用。
Dissociated neonatal rat retinal cells as testing cells were cultured on 96 well culture plates precoated with laminin.
新生大鼠视网膜的分离细胞,培养在包被基板蛋白的96孔培养板作为细胞检测系统。
Methods The human embryo Schwann cells were cultured for 2 weeks when they were seeded on polyglactin 910 scaffolds coating with rat tail glue and laminin together.
方法应用鼠尾胶和层粘蛋白包埋纤维状的聚羟基乙酸和聚乳酸共聚物支架与人胚雪旺细胞培养2周。
Methods The rat models of hepatic fibrosis were established by human serum albumin(HSA) injection. Serum TNF was detected by RIA method and laminin in the liver was studied by immunohistochemistry.
方法采用以人血白蛋白攻击方法制备肝纤维化动物模型,放免法测定血TNF ,免疫组化法观察肝内层粘蛋白改变。
Methods The rat models of hepatic fibrosis were established by human serum albumin(HSA) injection. Serum TNF was detected by RIA method and laminin in the liver was studied by immunohistochemistry.
方法采用以人血白蛋白攻击方法制备肝纤维化动物模型,放免法测定血TNF ,免疫组化法观察肝内层粘蛋白改变。
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