• Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.

    方法膜片细胞记录测量培养大鼠海马神经元钾离子电流

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  • Objective To improve the primary cell culture methods of colorectal adenomas.

    目的对大肠腺细胞培养方法作进一步的改进

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  • Primary cell culture was made from 23 malignant trophoblastic tumors and 19 normal early pregnant villi.

    本文23例恶性滋养细胞19例正常早孕绒毛滋养细胞进行原代培养

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  • We also established the cell line of this transgenic mice model through primary cell culture to make use this model more efficiently.

    为了更为有效地利用模型小鼠我们通过培养建立了该模型肿瘤细胞系

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  • Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.

    目的建立快速有效绒毛滋养细胞体外培养方法以及建立滋养细胞株初步评价

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  • Methods: Primary embryonic rat cortical neurons culture, cytotoxicity assays and biochemistry detection, cell stain for the neuron morphology in microscopy and image analysis.

    方法代胚胎皮质神经元细胞培养细胞毒性试验生化指标测定,细胞染色显微镜下观察形态学改变影像分析

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  • Objective: To further investigate the mechanisms of hair cell generation or regeneration, the primary culture systems of cochlear sensory epithelial cell (CSEC) of rats were established.

    目的建立耳蜗感觉上皮细胞(CSEC)培养体系,为研究毛细胞再生的分子机制提供大量来源一致的细胞。

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  • Objective To observe the changes of limbal stem cell morphology? Antigen and proliferative ability after primary culture in vitro.

    目的探讨人角膜缘干细胞体外培养细胞形态学、抗原性增殖能力变化

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  • NECL2 distributed in cell body and protuberance of primary culture neuron. The rat adrenal gland tumor cell line PC12 can be induced into neuronal differentiation.

    肾上腺嗜铬细胞瘤细胞PC12具有神经元分化潜能,PC12细胞中转染并过量表达NECL2能够诱导细胞发生神经元样分化。

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  • To establish the method on the primary culture of porcine intestinal epithelial cell, different digestion protocols and explant culture were used to identify the best method.

    分别采用消化法组织块培养建立小肠黏膜上皮细胞代培养方法

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  • We further developed the primary cell culture if the tumor cells which would lead to better use of this genetic model.

    同时建立培养肿瘤细胞提升了模型利用效率。

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  • Objective To study the inhibition of lung cancer cells of primary culture and A549 cell line on the proliferation of tumor-infiltrating lymphocytes (TIL) in vitro and its clinical implications.

    目的研究肺癌细胞细胞系a549肿瘤浸润性淋巴细胞抑制作用及其临床意义

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  • Methods: Using primary nerve cell culture, we observed the survival and growth of spinal cord neurons with phase-contract microscope.

    方法:胚胎大鼠脊髓神经细胞培养,倒置相差显微镜下进行细胞记数显微测量,观察神经元存活生长分化状况。

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  • METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.

    方法体外细胞培养MTT (噻唑蓝)比色,材料直接接触

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  • The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.

    消化法进行表皮细胞成纤维细胞培养,绘制表皮细胞生长曲线MTT法测定传代后表皮细胞的增殖。

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  • The primary culture of vascular smooth muscle cell used attachment-block method.

    血管平滑肌细胞培养采用法进行

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  • However, the primary cell culture methods which are employed now have many defects.

    然而目前正被应用滋养层细胞分离、培养、纯化方法存在一定程度的缺陷。

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  • Objective: to establish a human hepatocellular carcinoma cell line EHH1 by primary culture and to investigate its biological characteristics.

    目的:采用原代培养的方法建立肝癌细胞系ehh1生物学特性进行分析。

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  • The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.

    移植后肿瘤细胞形态生长分泌CA12 5功能方面均与原细胞株保持一致

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  • The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.

    移植后肿瘤细胞形态生长分泌CA12 5功能方面均与原细胞株保持一致

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