Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Test results are as follows: (1) Potato PCR amplification reaction system and procedure are be fixed by optimizing PCR reaction system and selecting suitable primers.
试验过程得到如下结果：(1)对马铃薯PCR反应体系进行了优化，筛选出合适的引物，确定马铃薯P CR反应体系和扩增程序。
This real-time PCR reaction system is highly specific, reliable, high sensitivity for detecting pathogenic fungus which could be useful for the diagnosis of clinical fungus infection.
建立的实时荧光PCR方法能特异、灵敏的检测临床病原真菌，为临床真菌感染的诊断提供了一种快速、准确的方法。
Method the canine multiplex system were used for PCR reaction and the product were analyzed by ABI310 genetic analyzer.
方法用建立的犬复合扩增体系进行PCR反应，用abi310型遗传分析仪对扩增产物进行检测。
The new rapid determination techniques were polyase chain reaction method (PCR), volt-ampere biosensor method, automatic microbe system method (AMS), improvement of MRS culture medium and ATP etc.
新的快速检测技术有聚合酶链式反应(PCR)技术、伏安型生物传感器、自动微生物检测系统(ams)、改良MRS培养基和三磷酸腺苷(atp)法等，较传统方法迅速、准确。
A temperature control system for quantitive polymerase chain reaction (PCR) is presented in the paper with both software and hardware configuration.
从硬件和软件两方面定量pcr反应温度控制系统的设计。
Furthermore, the reaction system and program of ERIC-PCR method for the acid mineral drainage were optimized.
确定了ERIC方法用于酸性矿坑水样品时的最佳扩增体系和反应程序。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35s promoter heterologous template for the detection of genetically modified maize was developed in this study.
建立了玉米转基因成分中35s启动子非同源模板的竞争定量pcr检测系统。
The linear extension of the second and three rounds of nested amplification had been listed separately in improved system, it would increase the specificity and efficiency of PCR reaction.
主要是将第二、三轮的嵌套扩增中的线性延伸单独列出，从而增加了反应的特异性和高效性。
The linear extension of the second and three rounds of nested amplification had been listed separately in improved system, it would increase the specificity and efficiency of PCR reaction.
主要是将第二、三轮的嵌套扩增中的线性延伸单独列出，从而增加了反应的特异性和高效性。
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