Methods A pair of primer was designed and a mismatch nucleotide was introduced in its upstream primer.

方法设计一对寡核苷酸引物，其上游引物为突变引物。

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Methods A pair of primer was designed and a mismatch nucleotide was introduced in its upstream primer.

方法设计一对寡核苷酸引物，其上游引物为突变引物。

youdao