The mutated gene was cloned by map-based cloning, and was shown to encode an SPP.
图位克隆表明一个编码SPP的基因发生突变。
This study provides a basis for map-based cloning and functional analysis of the target gene.
本研究为该基因的图位克隆及功能解析奠定了基础。
Abstract: the high-density markers are necessary for map-based cloning of rice genes, but the available marker could not reach the satisfying level.
摘要在水稻功能基因的图位克隆中，常需要高密度的分子标记，而目前公布的各种标记的密度还远未达到令人满意的程度。
These results laid an important foundation for fine mapping, map-based cloning and marker-assisted selection breeding of watermelon flesh firmness gene .
研究结果为控制西瓜果肉硬度基因的精细定位、图位克隆及分子标记辅助选择育种奠定了基础。
To understand the molecular basis of this QTL, we isolated the SKC1 gene by map-based cloning and found that it encoded a member of HKT-type transporters.
为了研究该QTL的分子基础，我们通过图位克隆的方法分离到了SKC1基因，发现它编码一个HKT型转运蛋白。
In this dissertation we focused on the function of genes associated with anther and pollen development including map-based cloning of Arabidopsis genes from male sterile mutants.
本论文以正向遗传学的方法研究花药及花粉发育过程的基因功能，主要利用图位克隆的方法从拟南芥雄性不育突变体中克隆相应的基因。
The map makes effective tools for QTL analysis, marker-assisted selection (MAS) in breeding programs and cloning of the genes responsible for various phenotypes (map-based cloning).
该图谱的建立为进行基因定位、分子标记辅助选择抗旱性育种和抗旱相关基因的图位克隆奠定基础。
This paper reviews establishment of introgression lines from wild tomato in Lycopersicon by MAS and application of theses lines in fine mapping, analysis of QTL effect and gene map-based cloning.
本文综述了野生番茄单片段渐渗系的创建及其在基因精细定位、QTL遗传效应分析以及图位克隆中的应用。
However the former cloning methods such as map-base cloning or transposon tagging which based on forward genetics can only cloned one or several genes at the same time.
以往的基因克隆方法如图位克隆法、转座子标签法等大都是建立在正向遗传学的基础上，这些方法只适用于一个或少数基因，应该说是零敲碎打的策略。
However the former cloning methods such as map-base cloning or transposon tagging which based on forward genetics can only cloned one or several genes at the same time.
以往的基因克隆方法如图位克隆法、转座子标签法等大都是建立在正向遗传学的基础上，这些方法只适用于一个或少数基因，应该说是零敲碎打的策略。
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