A new method for on-line purification of samples with MALDI-TOFMS was established.
为此,本研究建立了MALDI—TOFMS测试中在线纯化蛋白质样品的新方法。
Objective To screen glioma's disease-related proteins in serum by liquid chip-MALDI TOF Mass Spectrometry.
目的方法利用液体芯片-飞行质谱技术进行血清中胶质瘤疾病相关蛋白质的筛选。
The repeated results of the same sample and different batches of culture have proved the reproducibility of the MALDI-TOF analysis.
同一批和不同批次重复分析的结果显示,方法的重复性和细菌特征性蛋白质谱图的重现性较好。
For instance, the support for analysis can be used in place of a metal sample plate for mass analysis such as MALDI-TOFMS or as a part thereof.
例如,本发明的分析用保持体可以替代MALDI-TOFMS等质谱分析用的金属制样品板或用作样品板的一部分。
The ability of magnetic beads to separate and enrich peptides from serum made it possible to detect peptide toxins when coupled with MALDI-TOF-MS.
最后是应用建立的方法检测人、牛和鼠血清中的芋螺毒素以及检测灵敏度的研究。
Utilizing MALDI-TOF-MS technology, SNP genetic markers in degraded biological material can be analyzed, which has important meaning in forensic medicine.
利用MALDI TOF MS技术可以分析降解检材的SNP遗传标记,这在法医学中将有着重要的意义。
Results of SDS-PAGE, CD and MALDI-TOF showed that the recombinant protein was obtained successfully with high purity and possessed demethylation activity.
SDS - PAGE、圆二色谱和MALDI - TOF质谱分析结果表明,成功获得纯度较高的重组蛋白,且具有去甲基化的活性。
The principle of electrospray ionization-mass spectrometry (ESI-MS) and matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) is described.
概述了电喷雾电离质谱(esi - MS)、基质辅助激光解析电离质谱(MALDI - MS)的原理。
NDH was identified and characterized with infrared (IR), MALDI-TOF mass, and synchrotron X-ray absorption spectroscopies (XAS), and with computer simulations.
用红外光谱MALDI-TOF质谱和同步X 射线吸收谱以及计算机模拟对NDH进行了鉴定和表征。
MALDI-TOF mass spectrometry can be used for rapid, accurate and sensitive discrimination and identication of food-borne pathogens, showing the useful significances.
应用MALDI-TOF分析方法可实现在细菌属、种、菌株和血清型水平上对食品病原菌快速、准确地区分和鉴定,显示出广泛的应用前景。
Methods The differentially expressed proteins between HUVEC incubated with Cryptococcus and normal HUVEC were obtained by 2-D electrophoresis and identified by MALDI-TOF MS technology.
方法利用二维凝胶电泳获得新生隐球菌孵育后血管内皮细胞与正常细胞差异表达蛋白质点,并对部分差异蛋白点进行质谱鉴定分析。
Methods: The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification.
方法:以TRF1抗体应用免疫共沉淀方法,从细胞蛋白抽提物中分离TRF1蛋白复合物,并作蛋白质肽指纹谱鉴定;
Methods: The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification.
方法:以TRF1抗体应用免疫共沉淀方法,从细胞蛋白抽提物中分离TRF1蛋白复合物,并作蛋白质肽指纹谱鉴定;
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