Method:The indirect immunofluorescent assay was used in the present study.
方法：本研究采用间接免疫荧光法测定T细胞亚群；
Immunofluorescent assay were used to detect the distribution and phenotype of macrophages in the tumor tissues.
免疫荧光法观察瘤组织中巨噬细胞的分布及表型特征。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
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