But all the calli induced by immature embryos did not differentiate the embryoids through solid and liquid subculture.
但幼胚诱导的愈伤组织经固体继代培养和液体悬浮培养后,都没有胚状体的分化。
The difference from the gene type of wheat is an importance factor effecting on the one-step culture for immature embryos of wheat.
基因型差异是影响小麦幼胚一步成苗培养的主要因素。
Influence of different mass concentration of 2, 4-d on callus induction in maize inbred line 4112 from immature embryos with MS being used as basal culture medium was studied.
以玉米自交系4112的幼胚为外植体,以MS为基本培养基,研究了不同质量浓度的2,4 - D对愈伤组织诱导的影响。
With this medium, soft, transparent callus can be induced from immature embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2-6%.
以该配方诱导幼胚,处于胚胎选择阶段和组织与器官分化阶段的幼胚,能诱导出松软、晶莹透明的愈伤,诱导频率为2-6%。
The callus texture modification, establishment of suspension cell lines and plant regeneration were systematically studied with immature embryos from Chengdan15,18 genotypes in maize.
作者利用成单玉米15,18号的幼胚诱导的愈伤组织,系统地研究了愈伤组织的改良、悬浮细胞系的建立及植株再生。
Wheat genotypes Yangmai 158, Yangmai 10 are excellent transformation materials. Wheat immature embryos with 4 days preculture have high tissue culture response and strong regeneration capacity.
小麦基因型扬麦158、扬麦10是优良的转化材料,以预培养4天的小麦幼胚为受体,培养力高,再生能力强。
After being cultured 50-70 days, embryos (including mature and immature ones) as well as seedlings from the mature embryo are obtained from 7 crosses and 2 selfed cultivars with a certain frequency.
培养50—70天,7个杂交组合及2个自交品种的胚珠均获得一定频率的胚(包括成熟和不成熟胚)及成熟胚萌发的苗。
Somatic embryogenesis was successfully done in Liriodendron hybrids with the immature zygotic embryos as explants.
以杂交鹅掌楸幼胚为外植体,进行体细胞胚胎发生研究。
Somatic embryogenesis was successfully done in Liriodendron hybrids with the immature zygotic embryos as explants.
以杂交鹅掌楸幼胚为外植体,进行体细胞胚胎发生研究。
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