At present, the key problem is focused on the selection of what kind of reporter gene probe would be.
当前,报告基因显像的焦点是选择何种报告基因探针进行基因显像。
The invention can not only make use of the nontoxic gene marker as gene probe, but also set foundations for further clone of the gene through physical mapping.
本发明不仅可利用该无毒基因的标记作探针,而且为进一步通过物理作图法克隆该无毒基因奠定了基础。
In this article we introduced the principle, development and application in food microbiological detection of three common molecular biology methods, gene probe, PCR and gene chip.
我们主要介绍了基因探针检测法、PCR检测法和基因芯片检测法的原理、开发及其在食品微生物检测中的应用。
Each probe binds to the RNA of a specific gene-the one that contains a complementary set of chemical units, or bases.
每一个探针与一种特定基因的RNA绑定——它含有了一整套化学物质和基质的集合。
Objective: to probe into thinking and prospect of application of gene chip technique to research of the channel-viscera correlativity.
目的:探讨基因芯片技术在经脉脏腑相关研究中可能存在的应用思路及前景。
Objective to probe the problems that should be paid attention to in cochlea implantation for inherited deafness patients with EVA and to detect the SLC26A4 gene mutation underlying the disease.
目的分析伴前庭水管扩大(eva)的遗传性耳聋患者SLC26 A 4基因的突变特点,探讨该病行人工耳蜗植入时应注意的问题。
Conclusions: Using PCR amplification products to prepare gene chip probe was a quickly, simple effective method.
结论:利用PCR扩增产物制备基因芯片探针是一种快速、简便、实用的方法。
Based on the gene sequence of circumsporozoite protein(CSP) of Plasmodium vivax , the PCR/DNA probe detection system was prepared.
根据间日疟原虫环子孢子表面蛋白(CSP)基因序列研制了PCR/DNA探针检测系统。
Nanoparticle tagging DNA probe, which combines of nano-technology, gene project and DNA detection, is the focus of recent research field.
纳米粒子标记DNA探针的研究是纳米技术、基因组学、DNA检测等学科交叉的研究热点。
It is the first report of obtaining partial sequences of plant disease resistance gene by using antibody probe in the world, which provides a basis of cloning the whole gene encoding API protein.
在国内外首次利用抗体探针获得了植物抗病蛋白编码基因的部分序列,为进一步克隆全长的API蛋白基因奠定了基础。
Methods HCV RNA positive samples were analyzed by both gene chip and line probe assay in a comparative analysis.
方法对HCVRNA阳性血清用基因芯片法和线性探针法进行基因分型。
In this paper, the classification, manufacture, labelled methods and principle of detection of nanoparticle tagging DNA probe and its application in gene detection were described and reviewed.
本文从纳米粒子的分类、制备、标记和检测原理等方面对纳米粒子标记DNA探针的制备和应用进行了分析与评述。
Because the probe of 517 rifampin-resistant did not be dotted on the gene chip, only sites of 518 and 526 mutations were detected, which were consistent with the results of DNA sequencing.
另有两株为517和518、526和517位双位点突变,因为芯片上未点517位突变的探针,所以只检测到518和526位的突变,该突变与测序结果完全一致。
Objective to investigate the association between G72 gene polymorphisms and depression, and to probe the difference of G72 gene polymorphisms between depression with and without mixed family history.
目的探讨抑郁症与G72基因多态性的关系,以及是否有混合家族史的抑郁症其g72基因多态性有无区别。
The present invention is used as probe and PCR amplification detection sequence for gene chip diagnosis for candida albicans.
本发明作为基因芯片诊断的探针及PCR扩增检测序列,用于白色念珠菌的诊断。
The result showed that the probe above could effectively detect the SLP gene with a certain specificity.
试验结果表明,设计的寡核苷酸探针具有一定的特异性,能很好的检测乳酸杆菌中SLP基因。
Conclusion PNA combining with the DNA of target sequence had a highly specificity. Choosing PNA as the probe may improve the determined specificity of piezoelectric gene sensor.
结论PNA与靶序列dna结合有高度的特异性,选用PNA作探针,可提高压电基因传感器检测的特异性。
This paper reports about preparation and purification of ONA probe related to deoxycytidine kinase gene. The hybridization condition has been explored.
本文报道乳酸杆菌脱氧胞苷激酶基因的DNA探针的人工合成和分离纯化以及杂交条件的探讨。
Objective To probe into the angiotensin converting enzyme(ACE)gene polymorphisms and the risk factors in coronary heart disease(CHD) in the Hainan Li and Han nationality.
目的探讨海南黎汉族冠心病(CHD)血管紧张素转换酶(ACE)基因多态性与危险因素的关系。
Standard Affymetrix probe set annotation is gene-centered, i. e. probe set is precisely linked to gene, and probe set expression is interpreted as gene expression.
标准的探针集注释是以基因为中心,也就是将探针集与特定的基因关联起来,而且探针集的表达谱最终会被翻译成基因表达谱。
Objective to probe into the mechanisms of bone morphogenetic protein-2 (BMP-2) in osteosarcoma and to provide basis for gene therapy.
目的从分子水平探讨骨形态发生蛋白2 (BMP - 2)基因对骨肉瘤生物学行为的影响,并为骨肉瘤的基因治疗提供理论依据。
To develop a fluorescent MGB probe real time PCR platform for detection the mutation of phenylalanine hydroxylase gene in patients with phenylketonuria.
探讨荧光mgb探针实时PCR技术检测经典型苯丙酮尿症的基因突变。
Objective: to probe the effect of DangguiShaoyao-San on Calcitonin gene-related peptide (CGRP) and somatostatin (SS) of vascular dementia mouse.
目的探讨当归芍药散对血管性痴呆小鼠脑海马降钙素基因相关肽(cgrp)和生长抑素(SS)含量的影响。
Objective: to probe the effect of DangguiShaoyao-San on Calcitonin gene-related peptide (CGRP) and somatostatin (SS) of vascular dementia mouse.
目的探讨当归芍药散对血管性痴呆小鼠脑海马降钙素基因相关肽(cgrp)和生长抑素(SS)含量的影响。
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