Mice morula rate of parthenogenetic embryos in ethanol plus feeder cells group was highest 80.38%;
小鼠孤雌胚的桑椹胚率乙醇加饲养层组最高80.38 %；
Rat embryonic fibroblasts (REF) can be used as feeder cells in cultivating rat embryonic stem cells.
大鼠胚胎成纤维细胞(REF)可以作为培养大鼠胚胎干细胞的滋养层。
Mice blastocyst rate of parthenogenetic embryos in SrCl2 plus feeder cells group was highest 76.21%;
小鼠孤雌胚的囊胚率氯化锶加饲养层组最高76.21 %；
So the scientists in this study, employing a pharmacological treatment over 40 days, seeded feeder cells with human embryonic stem cells.
因此，科学家们在这项研究中，采用超过40天的药物处理，用人类胚胎干细胞种植饲养细胞。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
When cultured on feeder cells in the presence of many kinds of cytokines, primordial germ cells can proliferate continually and maintain undifferentiated state.
在饲养细胞层和多种生长因子的共同作用下，可保持原生殖细胞在体外不断增殖而不分化，最终建立EG细胞系。
Objective Adopt two methods to produce cells of feeder layer and compare and observe its result of culture to find a better method of culture.
目的采用两种方法制备饲养层细胞，对比观察培养效果，求得最佳培养方法，以期实现其有效利用。
Feeder layer cells and suitable culture medium that supplemented with cytokines of promoting proliferation or restraining differentiation were crucial to keep chicken es in an undifferentiated state.
为维持细胞在培养过程中的未分化状态，需要采用饲养层细胞培养，同时设计合理的培养液配方并添加多种抑制分化或促进增殖的细胞因子。
AIM: Feeder layer cells are the necessary condition to prevent the differentiation of embryonic stem cells cultured in vitro.
目的：为保持胚胎干细胞的高度未分化状态，饲养层细胞成为胚胎干细胞体外培养中不可缺少的条件。
Objective to explore the optimal condition of direct differentiation into dopaminergic neurons of embryonic stem cells in serum-free and feeder layer cell free medium.
目的寻求无血清、无饲养层细胞存在的情况下，胚胎干细胞向多巴胺能神经元定向诱导分化的最佳条件。
Human embryonic fibroblast feeder played a positive role during the ES cells isolation procedure.
人胚胎成纤维细胞饲养层在ES细胞分离过程中具有较好的作用。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
Conclusion: ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering.
结论：在复苏后早期阶段ES细胞培养中，不宜单独应用BRL条件培养基，须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层。
The feeder layers of bone marrow stromal cells allowed spermatogonial stem cells to proliferate well and remain undifferentiated.
结果原代的小鼠培养骨髓基质细胞能很好的维持精原干细胞的非分化性扩增。
Feeder system was superior to feeder-free system during the culture of ES cells.
在ES细胞培养过程中，有饲养层体系优于无饲养层体系。
Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.
目的：建立ICR小鼠胚胎成纤维细胞饲养层培养体系，用于胚胎干细胞建系及研究。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
应用推荐