Objective to construct expression plasmid of human motilin receptor.
目的构建人胃动素受体基因的原核表达载体。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
结论成功构建了重组人FHIT真核表达质粒。
Objective to construct eukaryotic expression plasmid for human insulin-like growth factor 1 (IGF-1).
目的构建人胰岛素样生长因子1 (IGF - 1)的真核细胞表达质粒。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
为构建其启动外源基因的质粒表达载体作准备。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
After mediated by eukaryotic expression plasmid-liposome, the fused protein was introduced into C2C12 cell system.
然后经真核表达质粒-脂质体介导，导入C2C 12细胞系。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
The sequence of the cloned SCF is same as the published report. The construction of expression plasmid is identified in a correct way.
序列分析表明所克隆的SCF序列与文献报道的一致，构建的表达载体经鉴定正确。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen (WHcAg) and prepare polyclonal antibodies.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
The adenoviruses and the eukaryotic expression plasmid have unique practicality in preparing tumor treating medicines and radiotherapy hypersensitivity.
该腺病毒和真核表达质粒在肿瘤治疗药物、放射治疗增敏的制备中具有独特的实用价值。
Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
The customers should provide the gene expression plasmid which has been done the fluorescence labelling. We will provide the detail reports and microscopical photos.
技术服务完成后向客户提供详细的实验报告和荧光共聚焦显微镜照片。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
In this dissertation, a partial fragment of NAD-SDH from apple fruit was cloned, the NAD-SDH antisense expression plasmid was constructed, and the antisense expression has been doing;
本研究克隆了苹果NAD-SDH 基因的部分cDNA 序列，构建了其反义表达载体，并正在通过农杆菌浸染法将该基因反义转化至蔷薇科植物中。
Objective To construct the eukaryotic coexpression plasmid encoding carcinoembryonic antigen(CEA) and heat shock proteins 70 (HSP70), then detect the expression of the plasmid in vitro.
目的构建癌胚抗原（CEA）与热休克蛋白70（HSP70）的真核双表达质粒，并检测其在体外的表达。
Conclusions a clone of murine GAD65 is successfully constructed. It is a basis step for further construction of expression plasmid that expresses GAD65 and for immunologic intervention of T1DM.
结论成功的克隆了小鼠gad65基因，为构建GAD65基因疫苗以及糖尿病的免疫干预治疗打下基础。
The discussion was mainly focused on the application of these detection methods, the problem result in the virus extraction progress, and the construction of expression plasmid having LSV CP gene.
重点讨论了各种检测方法在实际中的应用，百合无症病毒提取过程中需要解决的问题，外壳蛋白基因在大肠杆菌中表达载体的构建。
Expression products of recombinant plasmid mainly located in nucleus.
重组质粒表达产物定位于细胞核；
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
Objective To construct recombinant plasmid for expression of chymopapain in E.
目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS - 7细胞中表达。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
AIM: to construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP) -9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
目的：构建基质金属蛋白酶9靶向的RNA干扰质粒载体，观察其对小鼠巨噬细胞基质金属蛋白酶9基因表达的沉默作用。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
Objective To study the expression efficiency of CYP2J3 gene in rat smooth muscle cells (SMCs) by liposome mediated and naked plasmid transfection.
目的比较脂质体介导法和裸质粒直接转染法导入CYP2J3基因表达的效率。
Objective To study the expression efficiency of CYP2J3 gene in rat smooth muscle cells (SMCs) by liposome mediated and naked plasmid transfection.
目的比较脂质体介导法和裸质粒直接转染法导入CYP2J3基因表达的效率。
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