The substance are consists of: 1. initiation and maintenance of embryogenic calli;
其内容包括：（1）胚性愈伤组织的诱导与保存；
In Citrus, the embryogenic calli consisted of embryogenic and non-embryogenic cells.
长期继代培养的柑橘胚性愈伤组织由胚性细胞和非胚性细胞组成。
Many embryogenic calli were produced by subculture of calli induced from mature embryo of rice.
从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。
Somatic embryos could be induced after the embryogenic calli were transplanted on somatic embryo induction medium.
胚性愈伤组织转移到体细胞胚诱导培养基中可诱导出体细胞胚。
Rice chitinase gene was transferred into immature embryogenic calli of wheat using PDS1000/He particle delivery system.
利用PDS10 0 0 /氦气基因枪将水稻几丁质酶基因导入小麦幼胚盾片愈伤组织。
In the cells of non-embryogenic calli, ATPase located in vacuoles and involved in the function of the hydrolysis of vacuole.
非胚性愈伤组织细胞的ATP酶定位于液泡，与液泡的水解功能有关。
The results showed that the levels of endogenous phytohormones in embryogenic calli were higher than those in non-embryogenic calli.
结果表明，龙眼胚性愈伤组织中内源激素含量比非胚性愈伤组织高；
Using all the methods mentioned above, an efficient and stable transformation system of embryogenic calli of elite inbred line R18 was established.
通过以上各种条件的优化，本文建立了一套较为高效的玉米优良自交系R18转化及再生系统。
Using embryogenic calli from young embryos as explants, bean chitinase gene was transferred into spring wheat plants by microprojectile bombardment.
利用基因枪法，以菜豆几丁质酶基因转化小麦幼胚愈伤组织。
The ploidy detection indicated that the genotypes performed marked difference in the inducing rates of anther embryogenic calli and produced different results in ploidy detection of embryogenic calli.
结果表明，不同基因型荔枝花药胚性愈伤组织诱导率存在显著差异，胚性愈伤组织倍性检测结果也不同。
The ploidy detection indicated that the genotypes performed marked difference in the inducing rates of anther embryogenic calli and produced different results in ploidy detection of embryogenic calli.
结果表明，不同基因型荔枝花药胚性愈伤组织诱导率存在显著差异，胚性愈伤组织倍性检测结果也不同。
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