The recombinant DHFR protein containing his-tag was purified by using TALON metal affinity resin under nondenaturing conditions.
在非变性条件下，用TALON金属亲和层析树脂纯化含组氨酸标记的重组DHFR。
AIM: TO obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR-cells.
实现其在CHO - DHFR -细胞中的高效表达，获取生物活性较高的重组蛋白。
CONCLUSION: MTX induced-increase of recombinant antibody production in CHO cells can be increased by weakening the expression of DHFR gene.
结论：弱化表达载体中DHFR的表达，可提高MTX对工程抗体表达的增加效果。
The molecular mechanism of resistance to the antifolates has been well characterisied and is due to structural changes in the target enzymes resulting from the point mutations of DHFR or DHPS gene.
抗叶酸类抗疟药的抗药性机制已基本搞清，与其作用靶酶二氢叶酸还原酶或二氢蝶酸合成酶基因点突变相关。
The molecular mechanism of resistance to the antifolates has been well characterisied and is due to structural changes in the target enzymes resulting from the point mutations of DHFR or DHPS gene.
抗叶酸类抗疟药的抗药性机制已基本搞清，与其作用靶酶二氢叶酸还原酶或二氢蝶酸合成酶基因点突变相关。
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