Methods The whole-cell patch clamp with current clamp was used.
方法采用全细胞膜片钳技术,进行电流钳制。
Spontaneous discharge could be recorded by voltage clamp and current clamp.
在电压钳和电流钳下可记录到自发放电活动。
Recording AP in current clamp mode and recording current in voltage clamp mode.
AP记录采用电流箝方式,电流记录采用电压箝方式。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法:应用全细胞膜片箝电流箝制技术,对急性分离的大鼠DRG细胞进行电流箝制。
The overall effect in current clamp experiments was an increase in excitability as indicated by decreased rheobase and lower action potential threshold.
本实验观察到的整体效应为由于基电流降低和动作电位阈值下降而表现出的兴奋性增加。
Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法:运用全细胞膜片钳技术记录持续性钠电流,并观察其在缺氧-复氧模型下的变化。
This module uses a circuit topology of forward pulse width modulated - zero current quasi - resonance soft - switching , combined with active clamp technology.
该变换器电路结构上采用正激一零电流准谐振软开关电路拓扑结构,并结合了有源钳位技术;
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
Use clamp ampere meter to measure the current and check if the heating power is normal.
用钳形表测量电流,检查发热功率是否正常。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞,应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线(激活、失活、复活和去活化)。
By using an active clamp circuit, the power transformer core is symmetrically reset. Zero voltage switching conditions are achieved over a broad input voltage and output current range.
利用有源钳位电路实现主变压器铁心对称复位,功率开关在宽输入电压和宽负载电流范围内零电压开关。
Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.
以原代培养的大鼠海马神经元为标本,运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。
It describes the measure principle of the clamp grounded resister tester, that is, a effective close loop for current flow is necessary when doing the test.
介绍了钳形接地电阻测试仪的测试原理,即在测量时必须要有一个供电流流过的有效的闭合回路。
Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.
方法应用膜片钳技术全细胞构型从原代培养的螺旋神经元获得电流钳记录。
Methods: The patch clamp was used to record the changes in average whole cell current and the conductance of GABA-A receptor on cultured hippocampal neurons in different developing stages.
方法用膜片钳记录仪在体外培养的不同时期,记录大鼠海马神经元GABA-A受体离子通道全细胞通道电流、电导的变化。
Meanwhile, the clamp circuit will limit the output voltage or current at a fixed value when they are abnormal that caused by a DUT short circuit or other fault in order to protect the device.
当被测dut发生短路或其他异常导致电流或电压过高时,钳位电路会将输出电压或电流限制在一个固定值上,以保护器件不被损坏。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流,首先必须获得单个心肌细胞。
However if the active clamp flyback converter is designed to operate in CCM, and the duty cycle is controlled to make the input current track input voltage, the input power factor can be improved.
但如果设计反激有源箝位电路工作在电流连续模式,控制占空比,使输入电流跟随输入电压,可以提高输入功率因数。
Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果:(1)建立了膜片钳全细胞记录技术平台,获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
An infinite wire carrying standard high current perpendicular to the geometric center of the clamp ammeter should be the ideal method for the clamp ammeters calibration.
钳形电流表理想的校准方法是用标准电流源对一根无限长导线加测试电流,并使该导线垂直穿过被校钳形表的几何中心。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞,标准的全细胞膜片钳技术记录钠通道电流。
METHODS: Patch clamp technique (whole cell recording) was used to record L-Ca2 + current in single cardiac myocyte.
方法:用全细胞膜片箝记录心室肌细胞的L -钙电流。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
The input and output voltage ratings, VI and vo, may be exceeded if the input and output maximum clamp-current ratings, IIK and IOK, are observed.
如果观察输入和输出最大箝压电流额定值IIK和IOK,会发现输入和输出电压额定值VI和VO已经可能超过。
Methods Conventional microelectrode and patch clamp techniques were used to record the PTH-induced changes of action potential and pacemaker current If in the rabbit sinus node.
方法运用微电极、膜片箝技术,记录PTH对家兔自律性动作电位和兔起搏离子流的影响。
We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents.
采用全细胞膜片钳技术,记录动作电位和上述各离子流。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术,我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流(NSCC)。
Methods Enzyme digestion was performed to isolate single ventricular myocyte of guinea pig and whole-cell patch-clamp technique was used to record potassium current.
方法酶解法分离豚鼠单个心室肌细胞,应用全细胞膜片钳技术研究白藜芦醇对豚鼠单个心室肌细胞钾电流的影响。
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