The results showed that both the cell-free extract and culture supernate from mouse SRS-82 cell line were leukemogenic.
结果表明SRS-82 株的无细胞提取液和其培养上清液的离心沉淀物皆具有致白血病活性。
Methods Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surface of cementum treated with various methods.
方法应用体外细胞培养技术观察MC3T3 - E1成骨细胞株在不同处理的牙骨质表面的生长状况。
Conclusion: Under adequate culture conditions, high density and high expression culture of the recombinant cell line could be realized by using bioreactor.
结论：在较为适当的培养条件下，利用生物反应器可使工程细胞的培养达到高密度、高表达。
METHODS in vitro methylcellulose semisolid culture was used for the evaluation of clonal formation of K562 cell line. Flow cytometer was used for detecting apoptosis.
方法体外甲基纤维素半固体培养观察各处理组k 562细胞的克隆形成，流式细胞仪评价细胞的凋亡状况。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
We also established the cell line of this transgenic mice model through primary cell culture to make use this model more efficiently.
为了更为有效地利用该模型小鼠，我们通过原代培养建立了该模型的肿瘤细胞系。
Objective To study the inhibition of lung cancer cells of primary culture and A549 cell line on the proliferation of tumor-infiltrating lymphocytes (TIL) in vitro and its clinical implications.
目的研究肺癌原代细胞和细胞系a549对肿瘤浸润性淋巴细胞的抑制作用及其临床意义。
NECL2 distributed in cell body and protuberance of primary culture neuron. The rat adrenal gland tumor cell line PC12 can be induced into neuronal differentiation.
大鼠肾上腺嗜铬细胞瘤细胞PC12具有神经元样分化的潜能，在PC12细胞中转染并过量表达NECL2能够诱导细胞发生神经元样分化。
A culture method and assay for the rat small intestinal epithelial cell (IEC-6) line have been established and factors that influence the results of the culture have been investigated.
作者引进并建立了大鼠小肠上皮细胞(IEC—6)的培养传代和活性检测的实验方法；且对几种影响因素进行了探讨。
Excellent skill of cell culture, cell line proliferation, and anti-cancer drug screening.
能够良好的进行细胞培养，细胞增殖操作，并进行抗癌药物筛选。
Methods Induce the differentiation of erythroleukemia cell line HB60-5 to mature erythroid cells by altering the culture condition and plot a cell growth curve.
方法利用红白血病细胞株hb60 - 5的生长特性，通过改变培养条件，促使细胞向成熟细胞分化。
Aim: On-line analysis and control are very important for the optimization and achievement of high cell concentration and product yield in animal cell culture.
目的：在应用生物反应器进行动物细胞大规模培养时，过程的在线分析和调控技术对于高细胞密度和高产品得率的获得是非常重要的。
Murine bone marrow endothelial cell line could be established after long time culture and there is no significant reduction in their proliferation potency after 20 passages.
小鼠骨髓内皮细胞经过长期培养后，已转化为内皮细胞株，已传代20次，其增殖能力没有减退。
The conditions of isolation and culture of protoplasts from resistant Astragalus cicer L. cell line, Helianthemum Songaricum Schrenk and Zygophyllum xanthoxylum were studied.
对鹰嘴紫云英抗性系、半日花、霸王三种植物进行了原生质体分离和培养条件的研究。
The effects of genistein and daidzein on the proliferation of human breast cancer cell line in vitro were studied by cell culture and MTT colorimetric method.
应用体外细胞培养和MTT比色法。研究大豆异黄酮类化合物金雀黄素和大豆黄酮对人乳腺癌细胞系体外增殖作用的影响。
Objective: To culture and establish Chinese ethnic prostate cancer cell line, investigate its growth characterization and explore experimental methods and models of gene diagnosis and trial of drugs.
目的：培养建立中国人种前列腺癌细胞系，为了解中国人种前列腺癌细胞生长特性、进一步开展前列腺癌基因诊断和药物筛选建立方便的实验手段和模型打下基础。
Objective To study different methods of in vitro culture of human epithelial ovarian carcinoma, and the method of inducing drug-resistance cell line it.
目的研究人卵巢上皮癌体外培养的方法及其耐药株的诱导方法。
The effect of inducers on leukemia cell line K562 was studied in liquid culture system in vitro.
本文以人白血病细胞株k _(562)为实验模型，通过体外液体培养，研究了白血病细胞诱导剂的作用。
Objective: to establish a human hepatocellular carcinoma cell line EHH1 by primary culture and to investigate its biological characteristics.
目的：采用原代培养的方法建立一株人肝癌细胞系ehh1，并对其生物学特性进行分析。
The influence of KCl on the release of glutamic acid from culture human neuroblastoma cell line (SH SY5Y) was observed by using HPLC.
利用高效液相方法观察了氯化钾对培养人神经母细胞瘤细胞系(SHSY5Y)细胞谷氨酸释放的影响。
The method of explant culture was used successfully in this study to establish neonate goat intestinal epithelial cell(IEC)line.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.
移植后肿瘤细胞在形态、生长、分泌CA12 5功能方面均与原细胞株保持一致。
Methods:(1) Cell and cell culture Human gastric carcinoma cell line BGC823 was gifted from the Department of Typhoid of Nanjing University of Traditional ChineseMedicine.
细胞培养人胃癌细胞株BGC823由南京中医药大学伤寒教研室惠赠；
Results: in human osteosarcoma cell line MG-63 culture Type I collagen mRNA expression reach a maximum level on days 7.
结果：人成骨肉瘤MG- 63细胞株接种培养第7天后I型胶原基因表达较高。
Results: in human osteosarcoma cell line MG-63 culture Type I collagen mRNA expression reach a maximum level on days 7.
结果：人成骨肉瘤MG- 63细胞株接种培养第7天后I型胶原基因表达较高。
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