Methods: the mice were immunize with LOVO, and the mAb were screened by indirect cell ELISA and immunohistochemistry.
方法：用人结肠癌细胞株lovo免疫小鼠，用间接细胞ELISA和免疫组化的方法筛选抗结肠癌相关抗原的单抗。
Objective: to set up a method to measure 'cell surface sign with ELISA method.
目的：建立酶联免疫吸附法检测细胞表面标志的方法。
It was shown that the cell wall antigen can be used as antigen in ELISA as well as in plate agglutination text SAT.
试验证明，细胞壁抗原，既能用于传统的平板凝集试验和试管凝集试验，又能用于ELISA试验。
The fluorescence flow cytometry (FCM) DNA assay and TUNEL were applied to detect cell cycle phase and cell apoptosis. the endogenous VEGF contents were detected by ELISA method.
应用流式细胞术进行DNA倍体分析和TUNEL分析，检测两药单用与联用对细胞周期及早期凋亡的影响；
The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.
将纯化之球蛋白片段免疫 BALB/c小鼠后， 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1进行细胞融合。
The expression of VEGF in Tanshinone IIAtreated group was significantly lower than that in control group (P<0.01). The similar result could be observed in cell culture fluid with ELISA assay.
免疫细胞化学染色及ELISA法检测发现，丹参酮IIA作用组肝癌细胞VEGF表达和培养液中VEGF分泌量明显减少，与未加药对照组相比差异有统计学意义（P<0.01）。
Nasopharyngeal swabs taken from 328 neonates with pneumonia were analyzed by LCR-ELISA and cell culture. Results A method of LCR-ELISA for detection C. trachomatis infection was established.
采集328份肺炎新生儿鼻咽标本，分别对其进行培养和LCR扩增，应用ELISA检测有无沙眼衣原体感染。
The library was subjected to three rounds of positive and negative cell panning and enrichment and then it was selected by ELISA.
对初级抗体库进行亲和富集及ELISA筛选， 获得的阳性克隆进行免疫组化鉴定并测序。
The library was subjected to three rounds of positive and negative cell panning and enrichment and then it was selected by ELISA.
对初级抗体库进行亲和富集及ELISA筛选， 获得的阳性克隆进行免疫组化鉴定并测序。
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