AIM: To construct phage human anti idiotypic antibody library.
目的：构建噬菌体人源抗独特型抗体库。
Human monoclonal antibodies can be made by means of antibody library technology.
利用抗体库技术可制备人源单克隆抗体。
Objective To construct human phage antibody library and produce human monoclonal antibodies.
目的构建人噬菌体抗体组合文库，筛选人单克隆抗体。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.
结论：硫氰酸盐洗脱法用于噬菌体抗体的筛选有助于成功获得期望的高亲和力抗体。
Conclusion: Human genetic engineering antibody, which is able to react specifically to tetanus toxin, is obtained through screening phage antibody library.
结论：利用噬菌体抗体库技术筛选可得到与破伤风外毒素特异性结合的人抗破伤风类毒素基因工程抗体。
The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology.
噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法，在生物科学领域极具潜力。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library.
为了构建人源性抗脑胶质瘤抗体库，我们必须先从患者的血液内检测到抗脑胶质瘤抗体，才能为建立人源性噬菌体抗体库奠定基础。
Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体，为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
Phage displayed technology has become a powerful tool in preparing high affinity antibodies, and antibody library technology paves a way for obtaining high affinity antibodies.
噬菌体展示技术是一种制备高亲和力抗体的有力工具，抗体库技术则为筛选高亲和力抗体提供了良好的平台。
But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.
采用杂交瘤技术制备人源性单克隆抗体存在许多困难，90年代初期出现了抗体库技术，为抗体的制备提供了新的途径。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
Conclusion: the human single-chain antibody gene fragments were constructed successfully, which provides a way for constructing the human single-chain antibody library of anti-breast cancer.
结论：成功地构建了人源抗乳腺癌单链抗体基因片断，为进一步构建人源抗乳腺癌单链抗体库提供了试验基础。
A large human phage antibody library was subjected for panning with leukocytes from healthy donors and leukemia patients to select for specific antibodies against leukocytic surface proteins.
采用正常人和白血病患者的白细胞对人源噬菌体抗体库进行淘选，以获得对两种细胞表面蛋白特异的抗体。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
A library containing the genes that could be expressed as proteins would be referred to as a cDNA expression library, which could be screened for a protein that binds a particular antibody.
一个包含可表达为蛋白质的基因的文库称为cDNA表达文库，它可以用来筛选结合特定抗体的蛋白。
Monoclonal antibody is produced by immunizing animals with a synthetic acetyl-lysine peptide library.
该单克隆抗体用合成的乙酰化赖氨酸底物库肽段免疫动物制备。
The construction of phage library of scFV antibody.
人源性天然抗体库的构建。
The peptide's sequences that combine with it tightly can be screened from the peptides' library with the oligosaccharide monoclone antibody.
利用寡糖的单克隆抗体，从随机的肽文库中筛选出与之结合力较强的肽序列作为该寡糖的模拟肽。
Screening mimic peptide of antibody from polypeptides library is a wholly new thought of targeted therapy, which has shown an obvious technics superiority.
采用多肽文库技术从噬菌体肽库中筛选抗体模拟小肽则是一种全新的导向治疗思路。
By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists.
利用组合库和噬菌体展示技术，我们证明半抗原特异性抗体确实存在有外围载体的某些遗迹。
Objective To prepare polyclonal anti-LPS antibody with cross protection against LPS from different bacteria and to screen mimotopes of LPS from cyclic 7-mer phage peptide library.
目的获得具交叉保护性的抗细菌脂多糖（LPS）多克隆抗体与模拟LPS多表位的噬菌体展示环肽克隆。
Sequence analysis of the randomly picked 96 Ig clones showed the library contains various antibody gene types.
对随机挑取的96个克隆测序序列分析表明各种型别比例基本合适；
Sequence analysis of the randomly picked 96 Ig clones showed the library contains various antibody gene types.
对随机挑取的96个克隆测序序列分析表明各种型别比例基本合适；
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