PurposeThe aim is to purify human platelet factor 4 (PF4) using a monoclonal antibody affinity chromatography.
目的用单克隆抗体亲和色谱从人血小板破碎液中纯化血小板第4因子(PF4)。
To identify, quantify and annotate all human protein-coding genes, the HPP will use three tools: mass spectrometry, antibody affinity and bioinformatics.
为了识别、定量和注释所有的人类蛋白质编码基因,HPP将使用三种工具:质谱、亲和抗体、以及生物信息学。
Clonal algorithm is adopted to educe optimized service restoration strategy, through selecting and clone antibody with high affinity.
最后应用克隆算法得出了优化的故障恢复策略,选择亲和力高的抗体并进行克隆。
Because this method was to simulate the process of antibody maturation in vivo, the obtained antibodies was not only fully humanized but also with relatively high affinity.
该方法体外模拟了抗体亲和力成熟的过程,使得到的抗体不但可以完全的人源化,而且可以具有较高的亲和力。
The key of antibody-mediated immunological therapy is strong specificity of target antigen, low immunogenicity, high affinity of antibody and so on.
而抗体介导的免疫治疗的关键是靶抗原的特异性强,抗体的免疫原性低及亲和力高等。
Conclusion With the increase of the immunization times, the affinity of antibody come to mature and the proportion of dominant linear epitopes of BSA became higher.
结论抗体抗原亲和力随免疫次数的增加趋向饱和,BSA的线性表位为优势表位,随免疫次数的增加比重逐渐增强。
Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.
结论:硫氰酸盐洗脱法用于噬菌体抗体的筛选有助于成功获得期望的高亲和力抗体。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Phage displayed technology has become a powerful tool in preparing high affinity antibodies, and antibody library technology paves a way for obtaining high affinity antibodies.
噬菌体展示技术是一种制备高亲和力抗体的有力工具,抗体库技术则为筛选高亲和力抗体提供了良好的平台。
The residue activity of modified enzyme is 32.8% when the affinity to antibody disappear and the stability of modified enzyme is promoted obviously.
得到的修饰酶抗体结合能力消失,保留酶比活力32.8% ,酶的常温稳定性和热稳定性均有显著提高。
Typically, in previous reports, the purification of CP mainly relied on the antibody, liquid chromatography and affinity tag-based strategies.
在先前的研究中,CP的分离主要依赖抗体、液相色谱以及亲合标签纯化等技术。
The monoclonal antibody and polyclonal antibody were respectively purified from ascites and serum by affinity chromatography.
亲和层析分别纯化腹水和血清得到单克隆抗体和多克隆抗体。
The monoclonal antibody and polyclonal antibody were respectively purified from ascites and serum by affinity chromatography.
亲和层析分别纯化腹水和血清得到单克隆抗体和多克隆抗体。
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