经一次差速离心分别提取鸡胚尿囊液中的F_(48)E_8和N-79株鸡新城疫病毒(NDV),获得纯化抗原。
The Newcastle Disease viruses (NDV) of strains F48Eg and N-79 prepared from embryonated eggs were purified by one differential centrifugation.
方法:以鸡新城疫病毒(NDV)作为疫苗模型药物、乳酸-羟基乙酸共聚物(PLGA)为载体材料,采用W/O/W复乳-溶剂挥发法制备NDV-PLGA口服微球。
METHODS:Newcastle dis-ease virus(NDV)vaccine was used as model drug, and PLGA as carrying agent to prepare NDV-PLGA oral microspheres by W/O/W emulsifying-solvent evaporation technique.
贵州新城疫病毒分离物能够在鸡胚成纤维细胞上表现生长,引起特征性的细胞病变效应。
Guizhou isolate of Newcastle disease virus could grow on chicken embryo fibroblast and resulted in cytopathogenic effect.
病毒对1%的鸡红细胞无凝集作用,并在鸡胚内干扰了B_1系新城疫病毒的血凝素的形成;
The virus could not agglutinate 1% of the chicken erythrocytes and interfered with the development of the hemagglutinins by the B-1 strain of Newcastle disease virus in the chicken embryos.
应用裂解气相色谱法(PGC)分析了五株流感病毒和一株新城疫病毒感染的鸡胚尿囊冻干样品。
Five strain influenza and one strain Newcastle disease virus were analysed by pyrolysis gas chromatography (PGC).
通过鸡胚法和人工感染雏鸡测定其对新城疫病毒的抑制作用。
Inhibiting function of it to Newcastle Disease Virus (NDV) was tested through chicken embryos method and artificially infecting chickens.
该病毒凝集鸡红细胞的作用可被新城疫标准阳性血清所抑制,证明所分离的毒株为新城疫病毒。
The isolate revealed to be able to agglutinate chicken haemocytes with the agglutination reaction to be inhibited by Newcastle disease virus standard antiserum.
方法利用传统鸡胚培养方法制备新城疫病毒(NDV),通过PEG-6000沉淀,差速离心和蔗糖密度梯度离心等方法提取纯化了新城疫病毒(NDV)。
Methods Newcastle disease virus(NDV) was prepared in embryonic egg. NDV was purified by deposited of PEG-6000, speed centrifugation and sucrose—density gradient—centrifugation(SDGC).
方法利用传统鸡胚培养方法制备新城疫病毒(NDV),通过PEG-6000沉淀,差速离心和蔗糖密度梯度离心等方法提取纯化了新城疫病毒(NDV)。
Methods Newcastle disease virus(NDV) was prepared in embryonic egg. NDV was purified by deposited of PEG-6000, speed centrifugation and sucrose—density gradient—centrifugation(SDGC).
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