本实验室使霍乱肠毒素(CT)B亚单位基因在大肠杆菌中获得表达,并能分泌到胞外。
The recombinant plasmid pMM2 contained cholera enterotoxin B sub-unit gene CT-B were expressed in E. coli strain.
结果:散发性霍乱以无毒株为主,质粒及肠毒素基因的携带率极低,但耐药性有所增加。
Results: The sporadic outbreak of vibrio cholera has some characters: including non toxic strain infection, low detection rate of plasmid and ct gene and increase of drug resistance.
方法:采用血清学和分子生物学相结合的方法,研究深圳地区散发不同血清型霍乱弧菌的生化特性、质粒、肠毒素基因携带和耐药状况。
Methods: With serological and molecular techniques, we study chemical property, plasmid, CT gene and drug resistance of sporadic outbreak of vibro cholera in Shenzhen city.
方法:采用血清学和分子生物学相结合的方法,研究深圳地区散发不同血清型霍乱弧菌的生化特性、质粒、肠毒素基因携带和耐药状况。
Methods: With serological and molecular techniques, we study chemical property, plasmid, CT gene and drug resistance of sporadic outbreak of vibro cholera in Shenzhen city.
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