方法：采用生长曲线和集落形成实验。
Method: the cell proliferation curve and the clone forming approaches were adopted.
软琼脂集落形成试验和裸鼠成瘤试验。
Soft agar assay and nude mouse tumorigenicity assay were carried out.
转化细胞在软琼脂培养基有细胞集落形成。
The transformed cells were able to form colonies in soft-agar medium.
方法软琼脂集落形成试验和裸鼠成瘤实验。
Methods Soft AGAR assay and nude mouse tumorigenicity assay were used.
集落形成法检测细胞存活曲线，反映放射敏感性变化；
Cell survival curves were determined by colony formation method to evaluate changes of radiosensitivity of the cells.
通过细胞形态、软琼脂集落形成实验检测细胞的转化。
The cell growth and soft AGAR assay were run to test the transformed cells.
集落形成法结果显示，顺铂辐射增敏比为1.320；
它们的体外抗肿瘤活性通过MTT法和集落形成法进行了评价。
Their in vitro antitumor activity against human cancer cell lines were evaluated by MTT assay and colony formation assay.
烟草能显著地提高伤寒沙门氏菌突变率和CHO细胞集落形成率；
Tobacco increased significantly the mutation rate of Salmonella typhia and the formation rate of CHO colony.
并进行体外靶细胞结合实验、细胞毒性实验和集落形成抑制实验。
The two conjugates were proven to combine with osteosarcoma cell and in vitro cytotoxicity test and colony inhibition test were also performed.
并在半固体培养基中，观察其对人低密度骨髓细胞集落形成的刺激作用。
The enhancing effect of CKLF1 on the colony formation of human hematopoietic progenitor cells was identified in semi-solid culture.
SDH的活性随温度升高及时间延长而下降，与细胞集落形成能力有较好的相关性。
Results: SDH activity decreased with rising of temperatures, which was relevant to cell ability of forming colony.
造血祖细胞体外培养的研究表明，人参总皂甙能促进小鼠多种造血祖细胞的集落形成。
Hematopoietic progenitor cells culture also indicated that TSPG could enhance the colony formation of various hematopoietic progenitor cells in mice.
本文报道50例癌肿患者外用血t淋巴细胞集落形成能力和T淋巴细胞亚群分布的改变。
Changes of the ability of t lymphocyte colony formation and distribution of t lymphocyte subset of peripheral blood in 50 patients with malignant tumor was investigated.
对扩增的细胞进行免疫表型、定向分化、造血祖细胞集落分析及脾集落形成单位的研究。
The expanded cells were further analyzed by phenotype, committed differentiation, progenitor colony assay as well as colony forming unite spleen.
结果：细胞活力、细胞回收率、集落形成能力、集落回收率在冷冻的两组间差异不显著。
Results There were no significant differences between the two preserving methods in cell viability, cell recovery, clonogenic capacity, or colony recovery.
结论PF具有细胞免疫调节作用，同时对受照机体的脾集落形成细胞也有一定的保护作用。
Conclusion PF might act as an immune modulator and have a certain protective effect on colony-forming cells in spleen for irradiated organism.
目的：评价CD34 +细胞体外扩增时高增殖潜能集落形成细胞(HPPCFC)的变化。
Objective: To evaluate the changes of colony forming cells with high proliferative potential (HPP CFC) in human CD34 + cell culture after in vitro amplification.
目的观察不同方式抽取的新西兰白兔骨髓中成纤维细胞集落形成单位(CFU - F)数量差异。
Objective To observe the differences of colony forming unit-fibroblastic (CFU-F) Numbers in bone marrow aspirated in various ways from New Zealand white rabbits.
结果各组分离的细胞经体外培养均有集落形成，肝干细胞的表面标记提示形成的集落具有肝干细胞特性。
Results All isolated cells of each group grew as colonies in vitro. Their expression of specific markers suggested that they were hepatic stem cells.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
与原位缺口翻译实验相对应，集落形成试验表明，在高浓度醋酸棉酚或GTW作用下，细胞失去繁殖的能力；
In a colony-forming test, we found that the cells lost their proliferate ability, since no colonies could be formed, if gossypol or GTW were of high concentration;
现代医学认为真红患者的红系爆式集落形成单位(BFU - E)及红系集落形成单位(CFU - E)与正常人水平相近甚至更高一些。
Modern medicine has proved that BFU-E and CFU-E of PV is similar as or higher than normal level.
裸鼠处死时测量肿瘤重量，用半固体培养基进行粒—巨噬细胞集落形成单位(CFU - GM)实验并检测血白细胞、丙氨酸转氨酶和肌酐等。
When the mice were sacrificed, tumor weight was measured, and the assay of colony forming unit-granulocyte and macrophage (CFU-GM) was performed on semi-solid culture.
结果显示永生化胚胎肝细胞克隆形成率为31.2%，染色体核型分析表明细胞核型无明显异常，软琼脂集落形成试验表明细胞在软琼脂中不能生长。
It is showed that the cell clone-forming rat was 31. 2%. The immortalized human fetal hepatocytes had a normal karyotype and were not able to grow in soft AGAR culture.
方法：用碱性磷酸酶抗碱性磷酸酶法检测再障患者骨髓中T淋巴细胞表型的变化，并用半固体培养法检测再障患者骨髓及去T细胞后骨髓单个核细胞CFU-GM集落形成情况。
Methods: Phenotype of T lymphocytes in bone marrow and CFU GM yields of bone marrow mononuclear cells with or without T cells were assayed with APAAP and semi solid culture method, respectively.
结果：MAPK反义寡核苷酸可明显抑制细胞活力、蛋白质含量、集落形成和DNA合成，其抑制率分别为52。6%、50。5%、54。9%和47。5%，与对照组比较差异有显著性（P<0。01）。
Results The cell viability, protein content, colony formation and DNA synthesis were significantly inhibited by MAPK ASO, the inhibitory rate was 52.6% , 50.5% , 54.9% and 47.5% respectively(P< 0.01).
但是当它们在试管中生长时，相对于那些没有突变的对照细胞，那些具有突变的细胞生长更快，并能够形成更大的集落。
But when the cells were grown in a laboratory test tube, those with the mutation grew far faster and formed much larger colonies than control cells without the mutation.
同时，结果还显示，造血生长因子的联合应用可加速CFU-GM集落的形成。
The combination of HGFs may accelerate the formation of the colonies of CFU GM.
感染细胞(称作293-BK细胞)如其他293细胞一样生长，14天后可在软琼脂中形成细胞集落。
The infected cells (293-bk cells) grew as well as other 293 cells, and formed colony in soft AGAR 14 days later.
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