方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
结果显示不论DNA甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。
When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
采用聚合酶链式反应（PCR）技术对来自北京等地区的173份PCV2阳性样本的全长ORF2基因进行了扩增，并对扩增产物进行了限制性片段长度多态性（RFLP）分析。
Whole ORF2 genes of 173 positive clinical PCV2 samples from Beijing and other areas were amplified by PCR and followed by restriction fragment length polymorphism (RFLP) analysis.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法对110名重庆汉族个体，采用降落式PCR和限制性片段长度多态性法（RFLP）进行基因型分析。
Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing.
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
以甘蓝型油菜的28个基因组探针和两种限制性内切酶对包括46个中国品种、9个欧洲品种在内的59个甘蓝型油菜品种(系)的RFLP标记进行了分析。
RFLP patterns were analyzed from 59 cultivars of Brassica napus, including 46 Chinese and 9 European accessions, using 28 genomic probes of Brassica and 2 restriction enzymes.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
采用多聚酶链反应-限制性片段长度多态性法(PCR - RFLP)分析MGP和ALAD基因的多态性。
The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP.
用S基因聚合酶链反应-限制性片段长度多态性确定HBV基因型。
HBV genotypes were determined by RFLP based on S-gene PCR products.
目的 检测人脑胶质瘤中肿瘤浸润淋巴细胞 （TIL）是否存在抗原受体基因的限制性取用及其类型 ，探讨其免疫学和临床意义。
Objective To investigate the type and the limitary shared of T-cell receptor(TCR) genes in tumor-infiltrating lymphocytes(TIL) of glioma specimens obtained.
方法：选择符合入选标准的高血压患者336例，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法，进行ACE2基因分型。
Methods:336 hypertension patients were recruited in this trail. The distribution of ACE2 gene A9570G polymorphism was analyzed by PCR-RFLP in all participants.
方法：采用PCRSSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
方法应用聚合酶链反应限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。
MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese.
方法：采用PCR技术和限制性内切酶片段长度多态性（RFLP）的方法，检测中国北方汉族168个核心家系的基因型。
Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.
采用病例对照研究的方法，用聚合酶链反应(PCR)和限制性片段长度多态性分析(RFLP)研究TS患者与健康对照间DRD2P、COMT基因的基因型频率有无统计学差异。
DRD2P and COMT genotyping were carried out using PCR followed by RFLP and statistical analysis of genotype frequencies between ts patients and healthy controls was performed using SPSS programme.
限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.
限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.
应用推荐