方法建立大鼠内囊出血并应激性胃溃疡模型,测定血浆及胃组织匀装中垂体腺苷酸环化酶激活肽、血管活性肠肽含量。
Methods Establish a model of experimental gastric stress ulcers induced by internal capsule hemorrhage, and determine the levels of PACAP and VIP in plasma and stomach tissues by RIA.
目的比较两种红细胞超氧化物歧化酶(SOD)活性测定方法,以便在实际工作中根据不同的试验条件选取最合适的测活方法。
Objective Compare the two methods in measuring the SOD activity of mices erythrocyte in order to select seemly method for different conditions.
DNA饱和分析法,是一种简便的测定rna聚合酶相对含量和相对比活性的方法。
DNA saturation experiment is a convenient method for measuring the relative amount and specific activity of RNA polymerase.
中文摘要:目的:建立清醒自由活动大鼠脑内酪氨酸羟化酶活性的测定方法。
Objective: To set the measuring method of tyrosine hydroxylase activity in the brain of conscious rats.
利用化学发光分析技术建立一种新的一氧化氮合成酶(NOS)活性测定方法。
A new method for determining activities of nitric oxide synthase (NOS) was set by using chemiluminescent assay.
本研究测定了199个小麦品种全麦粉的小麦多酚氧化酶(ppo)活性及全麦粉中的多酚含量,进一步探讨了PPO活性测定方法。
In this study, 199 wheat varieties were measured to determine their variation in PPO activity and the polyphenol content. The method of polyphenol activity was also discussed.
本文介绍了用紫外分光光度计测定苹果梨花芽分化各时期叶片中IA A氧化酶活性的方法。
IAA oxidase activity during the fruit bud differentiation of "Ping Guo Li" in leaves measured by UV-Vis method was studied.
方法采用比色等方法测定32例窒息新生儿及30例正常新生儿血浆一氧化氮、一氧化氮合成酶活性水平。
Methods Levels of nitric oxide and nitric oxide synthase were measured by color matching method in 32 asphyxia neonatorums as well as 30 normal neonates.
血清醌还原酶(QR)、ALT、LDH和CK催化活性用酶动力学方法测定,肝丙二醛(MDA)含量按硫代巴比妥酸方法测定。
Serum quinone reductase (QR), ALT, LDH and CK activities were determined with enzyme dynamic methods. Liver malondialdehyde (MDA) content was measured by the method of thiobarbituric acid.
利用生化酶学方法,测定几种保护酶系在氧化环境下的活性变化。
Exploiting the method of biochemistry, we assay the activity of several protective enzymes in the oxidizing stress.
方法测定小鼠血液中超氧化物歧化酶和过氧化氢酶的活性。
Methods The activities of SOD and catalase in mouse blood were determined.
方法通过结扎双侧颈总动脉造成脑缺血模型,测定小鼠小肠的推进率及大鼠的胃酸分泌、胃蛋白酶的活性。
Methods the model was established by bilateral general cervical artery ligation mice intestinal motion rate, peptic acid secretion and the activity of pepsin were detected.
方法:应用一氧化氮(NO)及一氧化氮合酶(NOS)试剂盒测定巨噬细胞产生NO和NOS活性。
METHODS: The level of NO and NOS activity were measured by NO and NOS kits in cultured macrophages.
为此,我们在实验初期重新定义了酶活单位,并对纤维素酶的活性测定方法进行了优化,确定了适用于本菌株产生的纤维素酶活性测定的一种较优方案。
Therefor, in the beginning of this research the activity unit was redefined and the method for measuring the activity of cellulase produced by this strain was optimized.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
用端粒重复序列扩增法(TRAP)半定量方法测定细胞染毒前后端粒酶活性的变化;
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
重点研究了脂肪和尿素酶活性的不同测定方法的检测效果。
Specifically, it showed the different testing methods and their results of the fat and urease.
方法:对156例结核性胸腔积液和60例恶性胸腔积液标本进行腺苷脱氨酶测定,同时测定血清腺苷脱氨酶活性。
Methods: To assay the activity of ADA in specimen and serum between the patients of 156 tuberculous pleural effusions and 60 malignant pleural effusion.
方法采用端粒重复序列扩增文件-酶标法(TRAP-ELISA)测定尖锐湿疣皮损中端粒酶的活性,并与正常皮肤和恶性肿瘤对照比较。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
目的:建立重组TFPI-2抑制纤溶酶活性的测定方法。
Objective: To establish a assay for plasmin inhibition with the recombinant TFPI-2.
方法:运用端粒酶pcr -TRAP酶联免疫吸附试验(ELISA)测定法对30例肺癌患者及25例肺部良性疾病患者(对照组)的痰液进行端粒酶活性检测。
Methods: The sputa of 30 lung cancer patients and 25 benign lung disease patients (control group) were checked for telomerase activity with PCR-TRAP ELISA.
目的通过测定大鼠对氟西泮抗痫耐受性和依赖性时海马中一氧化氮合酶(NOS)蛋白表达及活性的变化,探讨一氧化氮(NO)在此过程中可能的作用。方法建立大鼠对氟西泮抗痫耐受性和依赖性的模型。
Aim To study the role of nitric oxide by measuring the expression and activity of nitric oxide synthase in hippocampus of rats tolerance and dependence to the anticonvulsant action of flurazepam.
方法采用紫外可见分光光度计活体测定酶的活性。
Methods The enzyme activities were measured in vitro with an ultraviolet-visible spectrophotometer.
方法:建立四氧嘧啶糖尿病小鼠动物模型,测定肝脏抗氧化酶、ATP酶活性、脂质过氧化产物、一氧化氮(NO)和肝糖原含量。
METHODS: the model of alloxan diabetic mice was established to determined the activities of enzymes of antioxidant and ATP, and the contents of MDA, NO and glycogenesis.
方法:采用MTT比色试验及酶动力学方法,测定PD L细胞的增殖和ALP活性。
Methods: MTT method and enzyme dynamics method were used to observe the ability of proliferation and ALP expression of PDL cells.
方法应用荧光测定法进行体外酶活性测定。
Methods Fluorometric assay was used to test the activity of the enzyme in vitro.
目的建立血管紧张素转化酶(ace)底物的合成方法,探讨血清ace活性的测定方法。
Objective To establish the synthetic method of the substrate for angiotensin-converting enzyme (ACE), and investigate the assay method of serum ACE.
采用人工接种和酶活性测定的方法,研究转入几丁质酶基因等1~4个抗真菌基因的13个转基因水稻株系对纹枯病的抗性和几丁质酶活性变化规律。
The research was conducted to reveal the regular of resistance to sheath blight and chitinase activities of 13 transgenic rice lines which modified by chitinase gene and other anti-fungal genes.
采用人工接种和酶活性测定的方法,研究转入几丁质酶基因等1~4个抗真菌基因的13个转基因水稻株系对纹枯病的抗性和几丁质酶活性变化规律。
The research was conducted to reveal the regular of resistance to sheath blight and chitinase activities of 13 transgenic rice lines which modified by chitinase gene and other anti-fungal genes.
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