转化子的阳性筛选率为 10 0 %。
应用微生物形态学和RAPD技术，对转化子的遗传背景进行鉴定。
With microbe morphologic and RAPD technique, we made a fringe identification of inherited background of the transformant.
经过文库检验，以邻苯二酚为显色指示剂从文库中筛选到阳性转化子。
After gene library tested, we screened positive transformants using catechol as color indicator.
结果表明：经预培养的转化愈伤组织中的荧光表达量明显高于未经预培养的转化子；
The results showed that the fluorescence expression of pre-cultured transformed callus was significantly higher than the transformed callus without pre-culture after the infection of Agrobacterium.
右击子单元列表，将会打开如图17所示的转化子单元弹出菜单。转化子单元弹出菜单如图17所示。
Right click on the subunit list table; this will open up a Convert subunits popup menu as shown in Figure 17 below.
对转化子进行摇瓶发酵研究，发酵终止时转化子GB0506的糖化酶活力比出发菌株F0410提高了17.5%。
Shake-flask fermentation under optimal conditions showed that glucoamylase secreted by the transformant GB0506 was 17.5% higher than parental strain F0410 at the end of fermentation.
经大肠杆菌转化后对转化子进行酶切验证，并将新构建的载体1305-3分别转入农杆菌菌株LBA4404和EHA105。
The new constructed vector, 1305-3, was transformed to the two agrobacterium strains, LBA4404 and EHA105, respectively.
用聚丙烯酰胺凝胶电泳技术对木霉菌的野生菌株T21及其4株REMI转化子T31、T34、T47、T55的可溶性蛋白和酯酶同工酶进行了比较研究。
The soluble proteins and esterase isozyme of Trichoderma wild strain T21 and REMI transformed strains T31, T34, T47, T55 were analysed with polyacrylamide gel electrophoresis.
用聚丙烯酰胺凝胶电泳技术对木霉菌的野生菌株T21及其4株REMI转化子T31、T34、T47、T55的可溶性蛋白和酯酶同工酶进行了比较研究。
The soluble proteins and esterase isozyme of Trichoderma wild strain T21 and REMI transformed strains T31, T34, T47, T55 were analysed with polyacrylamide gel electrophoresis.
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