这是欧李超低温保存成功的首例报道。
This is the first successful report of cryopreservation of in vitro Cerasus humilis.
藻类种质的超低温保存技术已受到广泛的重视。
The cryopreservation technique of algae germplasm has been appreciated extensively.
超低温保存或许是鲵鱼，又称隐鳃鲵的最后机会。
Cryopreservation may be the last chance for the hellbender, aka the snot otter.
超低温保存是一种有希望长期保存顽拗性种质资源的方法。
However, cryopreservation has been proved as an appropriate method for the recalcitrant seed germplasm long-term storage.
限制生长保存和超低温保存是植物种质离体保存的两种主要方法。
Restricting conservation and cryopreservation are two main methods of plant germplasm in vitro preservation.
由此，基本可以确认超低温保存未改变新疆野苹果试材的遗传稳定性。
Therefore, we could conclude that genetic stability of M. sieversii was not altered after cryopreservation.
内容摘要：液氮超低温保存是目前植物种质资源长期保存的理想方法。
Content: Cryopreservation has long been considered an important method for the long-term storage of plant germplasm.
在此条件下，分别在玉米苗期、拔节期、灌浆期采集叶片清洗后超低温保存。
In this condition, gathering cleaning leave at across seedling, elongation, filling stage. s and preserved in low temperature.
超低温保存预处理对茎尖细胞起到了一定的保护作用，冻后细胞壁依然完整。
The pretreatment before cryopreservation protected the cell and kept the cell wall to be completed after cryopreservation.
本文介绍了微藻保种的几种常用方法，包括继代保存、固定化保存和超低温保存。
Several kinds of typical methods of maintenance microalgae species are presented, including succeeding preservation, immobilization storage and cryopreservation.
但作为低温生物学中比较新的领域，超低温保存还有许多问题需要进一步研究探讨。
As a new research field in cryobiology, more problems of ultra-cryopreservation are needed to further study.
禾谷类作物胚性悬浮细胞系的建立及其超低温保存是现代植物生物技术中的基础工作。
Establishment and cryopreservation of Cereal cell suspension cultures are basic work in modern plant biotechnology.
鱼类胚胎由于其自身结构特征：体积大、含水量高、多室结构等，迄今超低温保存尚未成功。
Successful fish embryo cryopreservation has not been achieved due to its large volume, large content of water, complex multi-compartmental system, etc.
与对照相比，经超低温保存的样品新产生的甲基化位点有14个，而去甲基化的位点有22个。
Compared to the control, 14 sites showed denovo methylation, and 22 sites were demethylated in the samples after cryopreservation.
综上推测：使用丙二醇、甲醇作为抗冻剂以及玻璃化冷冻保存方法对真鲷心跳期胚胎超低温保存也许较为合适。
The use of methanol, 1,2-propanediol and vitrification in the cryopreservation of the red sea bream's embryos was advised.
水稻组织培养物超低温保存的效果常受到材料特性、冰冻保护剂、降温冰冻法、后处理及恢复培养等因素的影响。
Cryopreservation of tissue cultures of rice is usually influenced by some factors, such as material properties, cryoprotectant, freezing procedure, treatment after freezing and renewing culture.
超低温保存过程中所造成的冷冻损伤是制约鱼类胚胎超低温保存成功与否的关键，具体表现为渗透压影响、抗冻剂毒性、冰晶损伤等。
During the process of cooling and thawing, embryos are subjected to a series of cryoinjuries such as cold shock, ice formation, cryoprotectant toxicity and osmotic effect.
超低温保存过程中所造成的冷冻损伤是制约鱼类胚胎超低温保存成功与否的关键，具体表现为渗透压影响、抗冻剂毒性、冰晶损伤等。
During the process of cooling and thawing, embryos are subjected to a series of cryoinjuries such as cold shock, ice formation, cryoprotectant toxicity and osmotic effect.
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