重组质粒的转化及筛选；
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little applied on the monocotyledons plants.
来自受体菌自身的同源质粒，因克服了宿主的限制一修饰性，可以极显著地提高电转化效率。
A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided.
目的：建立一种高效、快速的大肠杆菌感受态细胞制备及质粒转化的方法。
Objective: To establish an efficient and rapid method for the preparation and transformation of competent cells from Escherichia coli.
枯草芽孢杆菌感受态细胞的质粒转化与质粒的分子构型有关。
The transformability of plasmid DNA in B. subtilis competent cell depends on its molecular structure.
应用花生胚小叶再生体系，以农杆菌介导法对质粒上的基因转化进行初步探讨。
Applying to leaflet regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research.
重组质粒PSP1，转化微生物，产生一种碱性蛋白酶vapk的方法。
Recobinant plasmid PSP1, microorganisms transformed therewith, and method for producing alkaline protease VAPK.
重组质粒pdsbcm，转化微生物，产生一种碱性蛋白梅vapk的方法。
Recombinant plasmid PDSBCM, microorganisms transformed therewith, and method for producing alkaline protease VAPK.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
酵母表达系统是在酿酒酵母质粒的发现和酵母转化技术的成熟基础上建立起来的真核生物表达系统。
Yeast expression systems are based on the discover of plasmid in Saccharomyces cere- visiae and success of yeast transformation.
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little apply on the monocotyledons plants.
将含突变基因的重组质粒转化大肠杆菌菌株bl2 1 (DE3)，经IPTG诱导表达获得的目的蛋白质均以可溶形式存在。
The recombinant plasmids containing mutant genes were transformed into the Escherichia coli strain BL21 (DE3), and the expressed proteins were found to be water soluble after the induction of IPTG.
目的研究氯化铷法制备感受态细胞的转化效率和不同条件对其的影响。方法采用氯化铷法制备感受态细胞，用不同的质粒进行转化。
Objective to investigate the transformation efficiency and affecting factors in the preparation of competent E. coli cells by using rubidium chloride (RbCl) method.
研究和分析了受体品种、质粒浓度对水稻花器介导法转化的影响。
Effects of receptor varieties, plasmids concentrations on the rice transformation rate via floral organ-mediated method were studied.
用发根农杆菌(agrobacteriumrhizogenes)的ri质粒转化药用植物牛膝(achyranthes bidentataBl。)，诱导出了抗卡那霉素的发状根。
Achyranthes bidentata BL, a famous medica plant, was transferred by ri plasmid with Agrobacterium rhizogenes, and hairy root with kanamycin resistance was induced successfully.
用发根农杆菌(agrobacteriumrhizogenes)的ri质粒转化药用植物牛膝(achyranthes bidentataBl。)，诱导出了抗卡那霉素的发状根。
Achyranthes bidentata BL, a famous medica plant, was transferred by ri plasmid with Agrobacterium rhizogenes, and hairy root with kanamycin resistance was induced successfully.
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