方法：可溶性蛋白质凝胶电泳。
方法可溶性蛋白质凝胶电泳。
目的：通过蛋白质凝胶电泳技术，寻找大鼠循经部位与非循经部位蛋白质条带的差异，证实循经部位是否存在特异蛋白。
Objective: By protein gel electrophoresis, to seek the differences between the meridian course and the non-meridian part in rat, to confirm whether there is any special protein in the meridian course.
所不同的是我们采用了优化的一维凝胶电泳和液相色谱串联质谱联合的复杂蛋白质混合物鉴定技术。
The difference of this chapter is that we use an optimized one-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry combined protein mixture identification technology.
用聚丙烯酰胺凝胶电泳法研究丝胶的蛋白质组成问题。
The protein composition of sericin have been studied by polyacrylamide gel electrophoresis.
采用SDS-聚丙烯酰胺凝胶电泳及银染法，对兔核移植胚胎蛋白质合成类型的变化进行了研究。
The qualitative patterns of protein synthesis in nuclear transplant rabbit embryos were examined by SDS-polyacrylamide gel electrophoresis followed by silver staining.
同时应用高效液相色谱和聚丙烯酰胺凝胶电泳（SDS-PAGE）两种分析技术研究染料结构对蛋白质标记的影响。
Two methods, high performance liquid chromatography and gel electrophoresis (SDS-PAGE), were used to evaluate the influence of different dyes'structures on protein labeling.
聚丙烯酰胺凝胶电泳显示章鱼干糖蛋白具有多糖和蛋白质的特征，但纯度不高。
PAGE electrophoresis showed the glycoproteins of dried octopus take on characteristic of polysaccharide and protein, and are not pure enough.
报道了改进的凝胶电泳分离后蛋白质溶液酶解方法。
An improved method of in-solution digestion of gel-separated proteins for mass spectrometry (MS) identification was developed.
用十二烷基硫酸钠-凝胶电泳(SDS -PAGE)和二维凝胶电泳鉴定蛋白质纯度。
The obtained proteins were checked for the purity through SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
诱导产物经12%的聚丙烯酸胺凝胶电泳进行检测，结果显示有分子量约为4.0KD的特异蛋白质条带出现，说明兔NP-1基因在大肠杆菌中得到成功表达。
The result of a some 4.0KD specific protein band appearing in 12% SDS-PAGE electrophoresis demonstrated that rabbit NP-1 gene expressed successfully in E.
一种在凝胶电泳前用来使蛋白质发生变性并用统一的电荷覆盖蛋白质的去污剂。
A detergent used to denature proteins and cover them in uniform charge before gel electrophoresis.
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS - PAGE)和毛细管区带电泳(CZE)分析蛋白质的组分。
The proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and capillary zone electrophoresis (CZE).
聚丙烯酰胺凝胶电泳实验结果表明在大约55kd的位置上有表达的蛋白质条带。
The result of polyacrylamide gel electrophoresis shows that the protein band of expression is found at the position of about 55kd.
目的：利用二维凝胶电泳法分离人肾组织磷酸化蛋白质组。
Objective: To separate human kidney phosphoproteome by two-dimensional gel electrophoresis.
采用SDS-聚丙烯酰胺凝胶电泳技术和电子显微技术，对银杏不同部位的营养贮藏蛋白质组分及其动态变化规律进行了研究。
The fraction of vegetative storage protein(VSP) in different parts of Ginkgo biloba and its annual dynamic changing rules were studied by the technology of SDS-PAGE and microscope.
采用SDS-聚丙烯酰胺凝胶电泳技术和电子显微镜技术，对银杏枝条营养贮藏蛋白质组分及其动态变化规律进行研究。
The components and dynamics of vegetative storage proteins(VSPs) in branches of Ginkgo biloba were investigated by SDS-PAGE and electronic microscopy.
研究蛋白质-蛋白质相互作用，由本地和变性凝胶电泳免疫印迹，免疫沉淀。
Study protein-protein interaction by native and denaturing gel electrophoresis, western blotting, and immunology precipitation.
结果：通过标志酶活力的监测，梯度离心后线粒体纯提高近12倍，线粒体蛋白质在双向凝胶电泳中得到很好的显现。
Results: the increase in purity of mitochondria was found to be 12 times by density gradient centrifugation. Mitochondrial proteins were displayed well in the 2 -...
本研究目的是建立高分辨率的小细胞肺癌细胞系NCI-H446细胞双向凝胶电泳图谱，并初步分析其蛋白质表达情况。
This study was to establish a well-resolved, reproducible 2-DE map of proteome in SCLC cell line NCI-H446, and analyze its protein profiles.
新的抗体连接方法是为蛋白质印迹法专门设计的。后者是一种凝胶电泳法可根据细胞和组织样本的分子重量对其进行分离。
The new antibody conjugates are designed for Western blotting applications, which use gel electrophoresis to separate proteins from a sample of cell or tissue based on molecular weight and size.
方法：可溶性蛋白质SDS - PAGE凝胶电泳。
通过双向凝胶电泳技术分析大鼠的肝脏蛋白质组变化。
The change of proteome of rat liver is analyzed by two-dimensional gel electrophoresis (2-DE).
双向凝胶电泳是蛋白质组学研究中的关键技术之一，涉及较多的实验步骤和试剂，常出现缺陷胶而导致实验失败。
Two-dimensional gel electrophoresis(2DE) is a key technology for proteomics, however, it often ended in defect gels in real experiments.
方法利用二维凝胶电泳获得新生隐球菌孵育后血管内皮细胞与正常细胞差异表达蛋白质点，并对部分差异蛋白点进行质谱鉴定分析。
Methods The differentially expressed proteins between HUVEC incubated with Cryptococcus and normal HUVEC were obtained by 2-D electrophoresis and identified by MALDI-TOF MS technology.
方法利用二维凝胶电泳获得新生隐球菌孵育后血管内皮细胞与正常细胞差异表达蛋白质点，并对部分差异蛋白点进行质谱鉴定分析。
Methods The differentially expressed proteins between HUVEC incubated with Cryptococcus and normal HUVEC were obtained by 2-D electrophoresis and identified by MALDI-TOF MS technology.
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