发酵液经过预处理后，依次用大孔树脂吸附，硅胶柱吸附层析和薄层层析等分离方法即可获得农用抗生素TS99纯品。
Pure TS99 could be obtained from pretreatment fermentation broth through macroreticular adsorbent, silica gel column chromatography and thin-layer chromatography.
采用硅胶、聚酰胺、葡聚糖凝胶LH-20柱层析法和制备薄层层析法对乙酸乙酯和正丁醇提取物进行了分离、纯化。
The ethyl acetate and n-butanol extraction were chromatographed on silica gel column, polyamide column, sephadex LH-20 column and preparing TLC, respectively.
盐穗木全草乙醇提取物，经萃取、硅胶柱层析、薄层层析的方法分离提取，并结合药敏实验对其进行效价测定。
The ethanol extract was subumitted to chromatography on silica gel column to isolate some compounds. We tested the potency of halostashys components with sensitivity test.
薄层层析采用的展开剂为正丁醇：乙酸：水 （4：2：1），层析板为硅胶板。
The TLC used the silicon sheet with the developing solvents n-butanol, acetic acid and water (4:2:1).
薄层层析采用的展开剂为正丁醇：乙酸：水 （4：2：1），层析板为硅胶板。
The TLC used the silicon sheet with the developing solvents n-butanol, acetic acid and water (4:2:1).
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