禾长蠕孢菌原生质体转化及突变体筛选与验证。
Transformation of HGE protoplasts and screening of the mutants.
本文着重介绍了难栽培食用菌原生质体融合技术，并概括了其发展前景。
The protoplast fusion technology of edible fungi culturing difficultly was introduced mainly, and the technology of future development was discussed.
报道了新抗生素M-90产生菌原生质体的制备、再生及对产抗生素的影响。
The paper reports the formation and regeneration of protoplast from the new antibiotics, Micromonospora M 90, as well as the effects on antibiotics yield producing strain.
本文还详细研究了菌龄、酶系统、酶解温度和时间等多种因素对这两种鸡纵菌原生质体形成和再生的影响。
While the effects of hyphal age, lytic enzyme system, osmotic stabilizer, enzymolysis temperature and time on the preparation and regeneration of protoplast were also discussed in this paper.
本文研究的是一种通过原生质体融合技术构建出的细胞工程菌。
In this paper, the strain is cell engineering bacteria, which constructed by the way of protoplast fusion.
在原生质体形成及再生的最佳条件下制备原生质体，结果发现，SD- 142菌的原生质体形成率及再生率分别达到了86.8%和31.3%。
Under the optimum conditions, the rates of formation and regeneration of protoplasts of stain SD-142 had reached to 86.8% and 31.3% respectively.
研究了球形红假单胞菌与酿酒酵母菌的原生质体融合条件，并对融合子性能进行了测定。
The results of the protoplast fusion between Rhodopseudomonas spheroides and Sacchaaromyces cerevisiae were studied in this paper.
本文阐述了食用菌单核和同核原生质体的发现和作用以及在食用菌遗传研究上的应用前景。
This paper elaborates the discovery and effect of monokaryon and homokaryon protoplasts and their application in genetic and breeding research of edible mushroom.
为了提高在一般条件下不易产生分生孢子的雀稗麦角菌的产碱率，对原生质体诱变育种的条件和方法进行了探讨。
The feasibility of the protoplast mutagenesis for increasing productivity was studied on a Claviceps paspali strain, which was not easy to produce conidia under laboratory conditions.
研究结果表明，香菇原生质体分离诱变是一种很有应用价值的食用菌菌种选育方法。
The results indicated that protoplast formation and mutagenesis are the potential methods for strain selection and improvement in edible fungi.
对7号淀粉酶菌M1033的遗传背景进行分析，建立了其原生质体制备、转化的优化条件。
After Genetic background analysis of Streptomyces diastaticus No. 7 strain M1033, the modified conditions of M1033 protoplasts and transformation were established.
研究了利用珍稀食用菌茶树菇双核菌丝进行原生质体分离和再生，以及原生质体单核菌株的筛选过程。
The preparation of monokaryon protoplasts and the screening of protoplast-derived monokaryon strains are important parts in mushroom research and breeding.
为提高海南霉素产量，分别对其产生菌进行氮离子束注入、原生质体再生等处理，获得高产菌株2-7、Y81。
High producing mutants 2 7 and Y81 of hainanmycin were obtained by ion beam implantation or protoplast regeneration.
因而，原生质体制备和再生技术是禾长蠕孢菌转化与基因克隆技术的基础。
So it is a key step to establish an effective system of preparation and regeneration of HGE protoplasts.
目的：对柱晶白霉素产生菌SK-0 6的原生质体制备和再生的条件，以及再生菌的产生抗生素能力进行了研究。
AIM The condition of protoplast formation and regeneration of leucomycin (LM) producing strain SK-06 and the ability of antibiotic producing of regeneration strains was studied in this paper.
本文报告食用菌侧耳属四个种六个菌株原生质体分离与再生的试验结果。
This paper reports experimental results of protoplast preparation and regeneration with 6 strains which belong to 4 species within genus pleurotus.
农抗5102产生菌同源菌株9011和FR008的原生质体融合重组子SR7能产生1种两亲本所没有的抗细菌活性物质。
The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not.
农抗5102产生菌同源菌株9011和FR008的原生质体融合重组子SR7能产生1种两亲本所没有的抗细菌活性物质。
The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not.
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